Conditioned Medium of Mesenchymal Stromal Cells: A New Class of Therapeutics

N. V. Bogatcheva, M. E. Coleman

Research output: Contribution to journalReview article


Mesenchymal stromal cell (MSCs) represent a class of biologics with the prospects for employment as immunomodulatory, tissue-protective, and regenerative therapeutics. In parallel with cellular therapy, cell-free therapy based on MSC-secreted bioactive factors is being actively developed. MSCs secrete a variety of protein, peptide, RNA, and lipid mediators which can be concentrated, frozen, or even lyophilized without loss of activity, which gives them a certain advantage over cellular products requiring liquid nitrogen storage and infrastructure to revive frozen cells. This review (i) describes currently conducted clinical trials of cell-free products containing MSC secretome; (ii) summarizes main approaches to the generation and characterization of conditioned media concentrates and extracellular vesicle isolates; (iii) analyzes a variety of preclinical studies where effectiveness of secretome products has been shown; and (iv) summarizes current knowledge about secretome bioactive components obtained by analysis of in vivo models testing the therapeutic potential of the MSC secretome.

Original languageEnglish (US)
Pages (from-to)1375-1389
Number of pages15
JournalBiochemistry (Moscow)
Issue number11
StatePublished - Nov 1 2019


  • conditioned media
  • exosomes
  • extracellular vesicles
  • MSC
  • secretome

ASJC Scopus subject areas

  • Biochemistry

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