Conditions affecting the isolation of human umbilical cord blood CD34+ cells

Jamie Case, Alison Rice, Marcus Vowels

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The magnetic cell sorting (MACS) system was used to isolate CD34+ human umbilical cord blood (HUCB) stem cells (SC). Variables that might affect the detection of CD34+ cells, purity, enrichment, and yield of CD34+ cells, and the co-expression by CD34+ cells of CD38 and HLA-DR and their clonogenic capabilities were investigated. These variables were (a) separation technique [Ficoll, Percoll mononuclear cell (MNC) preparation] and (b) storage of HUCB [fresh, stored overnight (O/N) at 4°C or frozen]. There was no difference between MNC prepared for MACS CD34+ SC isolation by Ficoll or Percoll separation. The use of fresh, O/N, or frozen HUCB did not affect the isolation of CD34+ SC.

Original languageEnglish (US)
Pages (from-to)255-260
Number of pages6
JournalJournal of Hematotherapy and Stem Cell Research
Volume5
Issue number3
StatePublished - Jun 1996
Externally publishedYes

Fingerprint

Fetal Blood
Blood Cells
Ficoll
Stem Cells
Cell Separation
HLA-DR Antigens

ASJC Scopus subject areas

  • Immunology
  • Hematology

Cite this

Conditions affecting the isolation of human umbilical cord blood CD34+ cells. / Case, Jamie; Rice, Alison; Vowels, Marcus.

In: Journal of Hematotherapy and Stem Cell Research, Vol. 5, No. 3, 06.1996, p. 255-260.

Research output: Contribution to journalArticle

@article{d1bbeebf96df49fea4d7b104b5b239cc,
title = "Conditions affecting the isolation of human umbilical cord blood CD34+ cells",
abstract = "The magnetic cell sorting (MACS) system was used to isolate CD34+ human umbilical cord blood (HUCB) stem cells (SC). Variables that might affect the detection of CD34+ cells, purity, enrichment, and yield of CD34+ cells, and the co-expression by CD34+ cells of CD38 and HLA-DR and their clonogenic capabilities were investigated. These variables were (a) separation technique [Ficoll, Percoll mononuclear cell (MNC) preparation] and (b) storage of HUCB [fresh, stored overnight (O/N) at 4°C or frozen]. There was no difference between MNC prepared for MACS CD34+ SC isolation by Ficoll or Percoll separation. The use of fresh, O/N, or frozen HUCB did not affect the isolation of CD34+ SC.",
author = "Jamie Case and Alison Rice and Marcus Vowels",
year = "1996",
month = "6",
language = "English (US)",
volume = "5",
pages = "255--260",
journal = "Stem Cells and Development",
issn = "1547-3287",
publisher = "Mary Ann Liebert Inc.",
number = "3",

}

TY - JOUR

T1 - Conditions affecting the isolation of human umbilical cord blood CD34+ cells

AU - Case, Jamie

AU - Rice, Alison

AU - Vowels, Marcus

PY - 1996/6

Y1 - 1996/6

N2 - The magnetic cell sorting (MACS) system was used to isolate CD34+ human umbilical cord blood (HUCB) stem cells (SC). Variables that might affect the detection of CD34+ cells, purity, enrichment, and yield of CD34+ cells, and the co-expression by CD34+ cells of CD38 and HLA-DR and their clonogenic capabilities were investigated. These variables were (a) separation technique [Ficoll, Percoll mononuclear cell (MNC) preparation] and (b) storage of HUCB [fresh, stored overnight (O/N) at 4°C or frozen]. There was no difference between MNC prepared for MACS CD34+ SC isolation by Ficoll or Percoll separation. The use of fresh, O/N, or frozen HUCB did not affect the isolation of CD34+ SC.

AB - The magnetic cell sorting (MACS) system was used to isolate CD34+ human umbilical cord blood (HUCB) stem cells (SC). Variables that might affect the detection of CD34+ cells, purity, enrichment, and yield of CD34+ cells, and the co-expression by CD34+ cells of CD38 and HLA-DR and their clonogenic capabilities were investigated. These variables were (a) separation technique [Ficoll, Percoll mononuclear cell (MNC) preparation] and (b) storage of HUCB [fresh, stored overnight (O/N) at 4°C or frozen]. There was no difference between MNC prepared for MACS CD34+ SC isolation by Ficoll or Percoll separation. The use of fresh, O/N, or frozen HUCB did not affect the isolation of CD34+ SC.

UR - http://www.scopus.com/inward/record.url?scp=0029739221&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029739221&partnerID=8YFLogxK

M3 - Article

VL - 5

SP - 255

EP - 260

JO - Stem Cells and Development

JF - Stem Cells and Development

SN - 1547-3287

IS - 3

ER -