Confirmation of alcohol preference quantitative trait loci in the replicate high alcohol drinking and low alcohol drinking rat lines

Tatiana Foroud, Aimee Ritchotte, John Spence, Lixiang Liu, Lawrence Lumeng, Ting Kai Li, Lucinda G. Carr

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Objective: Selective breeding has been employed to develop replicate high-alcohol-drinking (HAD1 and HAD2) and low-alcohol-drinking (LAD1 and LAD2) rat lines from the heterogeneous N/Nih rat. Within-family selection and a rotational breeding design were used to discourage inbreeding (Li et al., 1993). A genome screen was previously performed using 459 HAD1 x LAD1 F2 progeny to identify quantitative trait loci (QTLs) on rat chromosomes 5, 10, 12 and 16 that contribute to alcohol preference and consumption in these non-inbred rat models of alcoholism. Methods: To confirm these QTLs in the replicate lines, 16 HAD2 and 16 LAD2 rats were genotyped for microsatellite markers within each of these QTL intervals. Results: Review of the genotypic data support confirmation of the QTLs on chromosomes 5 and 10; several markers in the QTL region display different alleles in the HAD2 and LAD2 rats, suggesting linkage disequilibrium between the microsatellite markers and the QTL. Although the QTL on chromosome 12 had the highest LOD score in the HAD1 and LAD1 studies, little evidence supported confirmation of this QTL based on the genotyped markers. Conclusions: Further evaluation of each of these QTL regions is ongoing in a sample of HAD2 x LAD2 F2 progeny currently being generated that will be used to assess the evidence of linkage in each of these QTL regions.

Original languageEnglish
Pages (from-to)155-161
Number of pages7
JournalPsychiatric Genetics
Volume13
Issue number3
DOIs
StatePublished - Sep 2003

Fingerprint

Quantitative Trait Loci
Alcohol Drinking
Alcohols
Chromosomes, Human, Pair 10
Chromosomes, Human, Pair 5
Microsatellite Repeats
Chromosomes, Human, Pair 16
Chromosomes, Human, Pair 12
Inbreeding
Linkage Disequilibrium
Alcoholism
Breeding
Alleles
Genome
Type 2C Congenital disorder of glycosylation

Keywords

  • Alcoholism
  • Linkage disequilibrium
  • Non-inbred rat lines
  • Quantitative trait locus

ASJC Scopus subject areas

  • Genetics(clinical)
  • Psychiatry and Mental health
  • Genetics
  • Neuroscience(all)

Cite this

Confirmation of alcohol preference quantitative trait loci in the replicate high alcohol drinking and low alcohol drinking rat lines. / Foroud, Tatiana; Ritchotte, Aimee; Spence, John; Liu, Lixiang; Lumeng, Lawrence; Li, Ting Kai; Carr, Lucinda G.

In: Psychiatric Genetics, Vol. 13, No. 3, 09.2003, p. 155-161.

Research output: Contribution to journalArticle

Foroud, Tatiana ; Ritchotte, Aimee ; Spence, John ; Liu, Lixiang ; Lumeng, Lawrence ; Li, Ting Kai ; Carr, Lucinda G. / Confirmation of alcohol preference quantitative trait loci in the replicate high alcohol drinking and low alcohol drinking rat lines. In: Psychiatric Genetics. 2003 ; Vol. 13, No. 3. pp. 155-161.
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