Congenital Asplenia in Mice and Humans with Mutations in a Pbx/Nkx2-5/p15 Module

Matthew Koss, Alexandre Bolze, Andrea Brendolan, Matilde Saggese, Terence D. Capellini, Ekaterina Bojilova, Bertrand Boisson, Owen W.J. Prall, David A. Elliott, Mark Solloway, Elisa Lenti, Chisa Hidaka, Ching Pin Chang, Nizar Mahlaoui, Richard P. Harvey, Jean Laurent Casanova, Licia Selleri

Research output: Contribution to journalArticlepeer-review

52 Scopus citations

Abstract

The molecular determinants of spleen organogenesis and the etiology of isolated congenital asplenia (ICA), a life-threatening human condition, are unknown. We previously reported that Pbx1 deficiency causes organ growth defects including asplenia. Here, we show that mice with splenic mesenchyme-specific Pbx1 inactivation exhibit hyposplenia. Moreover, the loss of Pbx causes downregulation of Nkx2-5 and derepression of p15Ink4b in spleen mesenchymal progenitors, perturbing the cell cycle. Removal of p15Ink4b in Pbx1 spleen-specific mutants partially rescues spleen growth. By whole-exome sequencing of a multiplex kindred with ICA, we identify a heterozygous missense mutation (P236H) in NKX2-5 showing reduced transactivation in vitro. This study establishes that a Pbx/Nkx2-5/p15 regulatory module is essential for spleen development.

Original languageEnglish (US)
Pages (from-to)913-926
Number of pages14
JournalDevelopmental Cell
Volume22
Issue number5
DOIs
StatePublished - May 15 2012

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology

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