Connective tissue growth factor is involved in pancreatic repair and tissue remodeling in human and rat acute necrotizing pancreatitis

Fabio F. Di Mola, Helmut Friess, Erick Riesle, Alexander Koliopanos, Peter Büchler, Zhaowen Zhu, David R. Brigstock, Murray Korc, Markus W. Büchler

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Objective: To analyze the involvement of connective tissue growth factor (CTGF) in the transforming growth factor-β (TGF-β) pathway during acute necrotizing pancreatitis (ANP) in humans and rats. Summary Background Data: Connective tissue growth factor is involved in several fibrotic diseases and has a critical role in fibrogenesis and tissue remodeling after injury. Methods: Normal human pancreas tissue samples were obtained through an organ donor program from five individuals without a history of pancreatic disease. Human ANP tissues were obtained from eight persons undergoing surgery for this disease. In rats, ANP was induced by intraductal infusion of taurocholate. The expression of CTGF was studied by Northern blot analysis, in situ hybridization, and immunohistochemistry in both human and rat pancreatic tissue samples. Results: Northern blot analysis revealed enhanced CTGF mRNA expression in human ANP tissue samples compared with normal controls. In addition, a concomitant increase in TGF-β1 was present. By in situ hybridization, CTGF mRNA was localized in the remaining acinar and ductal cells and in fibroblasts. In regions of intense damage adjacent to areas of necrosis, CTGF mRNA signals were most intense. Inflammatory cells were devoid of any CTGF mRNA signals. By immunohistochemistry, CTGF protein was localized at high levels in the same cell types as CTGF mRNA. In ANP in rats, concomitantly enhanced mRNA levels of CTGF, TGF-β1, and collagen type 1 were present, with a biphasic peak pattern on days 2 to 3 and day 7 after induction of ANP. Conclusions: These data indicate that CTGF participates in tissue remodeling in ANP. The expression of CTGF predominantly in the remaining acinar and ductal cells indicates that extracellular matrix synthesis after necrosis is at least partly regulated by the remaining pancreatic parenchyma and only to a minor extent by inflammatory cells. Blockage of CTGF, a downstream mediator of TGF-β in fibrogenesis, might be useful as a target to influence and reduce fibrogenesis in this disorder.

Original languageEnglish (US)
Pages (from-to)60-67
Number of pages8
JournalAnnals of Surgery
Volume235
Issue number1
DOIs
StatePublished - 2002
Externally publishedYes

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Acute Necrotizing Pancreatitis
Connective Tissue Growth Factor
Messenger RNA
Acinar Cells
Northern Blotting
In Situ Hybridization
Necrosis
Immunohistochemistry
Pancreatic Diseases
Taurocholic Acid
Transforming Growth Factors
Collagen Type I

ASJC Scopus subject areas

  • Surgery

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Connective tissue growth factor is involved in pancreatic repair and tissue remodeling in human and rat acute necrotizing pancreatitis. / Di Mola, Fabio F.; Friess, Helmut; Riesle, Erick; Koliopanos, Alexander; Büchler, Peter; Zhu, Zhaowen; Brigstock, David R.; Korc, Murray; Büchler, Markus W.

In: Annals of Surgery, Vol. 235, No. 1, 2002, p. 60-67.

Research output: Contribution to journalArticle

Di Mola, FF, Friess, H, Riesle, E, Koliopanos, A, Büchler, P, Zhu, Z, Brigstock, DR, Korc, M & Büchler, MW 2002, 'Connective tissue growth factor is involved in pancreatic repair and tissue remodeling in human and rat acute necrotizing pancreatitis', Annals of Surgery, vol. 235, no. 1, pp. 60-67. https://doi.org/10.1097/00000658-200201000-00008
Di Mola, Fabio F. ; Friess, Helmut ; Riesle, Erick ; Koliopanos, Alexander ; Büchler, Peter ; Zhu, Zhaowen ; Brigstock, David R. ; Korc, Murray ; Büchler, Markus W. / Connective tissue growth factor is involved in pancreatic repair and tissue remodeling in human and rat acute necrotizing pancreatitis. In: Annals of Surgery. 2002 ; Vol. 235, No. 1. pp. 60-67.
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T1 - Connective tissue growth factor is involved in pancreatic repair and tissue remodeling in human and rat acute necrotizing pancreatitis

AU - Di Mola, Fabio F.

AU - Friess, Helmut

AU - Riesle, Erick

AU - Koliopanos, Alexander

AU - Büchler, Peter

AU - Zhu, Zhaowen

AU - Brigstock, David R.

AU - Korc, Murray

AU - Büchler, Markus W.

PY - 2002

Y1 - 2002

N2 - Objective: To analyze the involvement of connective tissue growth factor (CTGF) in the transforming growth factor-β (TGF-β) pathway during acute necrotizing pancreatitis (ANP) in humans and rats. Summary Background Data: Connective tissue growth factor is involved in several fibrotic diseases and has a critical role in fibrogenesis and tissue remodeling after injury. Methods: Normal human pancreas tissue samples were obtained through an organ donor program from five individuals without a history of pancreatic disease. Human ANP tissues were obtained from eight persons undergoing surgery for this disease. In rats, ANP was induced by intraductal infusion of taurocholate. The expression of CTGF was studied by Northern blot analysis, in situ hybridization, and immunohistochemistry in both human and rat pancreatic tissue samples. Results: Northern blot analysis revealed enhanced CTGF mRNA expression in human ANP tissue samples compared with normal controls. In addition, a concomitant increase in TGF-β1 was present. By in situ hybridization, CTGF mRNA was localized in the remaining acinar and ductal cells and in fibroblasts. In regions of intense damage adjacent to areas of necrosis, CTGF mRNA signals were most intense. Inflammatory cells were devoid of any CTGF mRNA signals. By immunohistochemistry, CTGF protein was localized at high levels in the same cell types as CTGF mRNA. In ANP in rats, concomitantly enhanced mRNA levels of CTGF, TGF-β1, and collagen type 1 were present, with a biphasic peak pattern on days 2 to 3 and day 7 after induction of ANP. Conclusions: These data indicate that CTGF participates in tissue remodeling in ANP. The expression of CTGF predominantly in the remaining acinar and ductal cells indicates that extracellular matrix synthesis after necrosis is at least partly regulated by the remaining pancreatic parenchyma and only to a minor extent by inflammatory cells. Blockage of CTGF, a downstream mediator of TGF-β in fibrogenesis, might be useful as a target to influence and reduce fibrogenesis in this disorder.

AB - Objective: To analyze the involvement of connective tissue growth factor (CTGF) in the transforming growth factor-β (TGF-β) pathway during acute necrotizing pancreatitis (ANP) in humans and rats. Summary Background Data: Connective tissue growth factor is involved in several fibrotic diseases and has a critical role in fibrogenesis and tissue remodeling after injury. Methods: Normal human pancreas tissue samples were obtained through an organ donor program from five individuals without a history of pancreatic disease. Human ANP tissues were obtained from eight persons undergoing surgery for this disease. In rats, ANP was induced by intraductal infusion of taurocholate. The expression of CTGF was studied by Northern blot analysis, in situ hybridization, and immunohistochemistry in both human and rat pancreatic tissue samples. Results: Northern blot analysis revealed enhanced CTGF mRNA expression in human ANP tissue samples compared with normal controls. In addition, a concomitant increase in TGF-β1 was present. By in situ hybridization, CTGF mRNA was localized in the remaining acinar and ductal cells and in fibroblasts. In regions of intense damage adjacent to areas of necrosis, CTGF mRNA signals were most intense. Inflammatory cells were devoid of any CTGF mRNA signals. By immunohistochemistry, CTGF protein was localized at high levels in the same cell types as CTGF mRNA. In ANP in rats, concomitantly enhanced mRNA levels of CTGF, TGF-β1, and collagen type 1 were present, with a biphasic peak pattern on days 2 to 3 and day 7 after induction of ANP. Conclusions: These data indicate that CTGF participates in tissue remodeling in ANP. The expression of CTGF predominantly in the remaining acinar and ductal cells indicates that extracellular matrix synthesis after necrosis is at least partly regulated by the remaining pancreatic parenchyma and only to a minor extent by inflammatory cells. Blockage of CTGF, a downstream mediator of TGF-β in fibrogenesis, might be useful as a target to influence and reduce fibrogenesis in this disorder.

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