Connectivities of the striatal grafts and laminin guiding

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Abstract

This chapter discusses the current study of the chief afferents and efferents that participate in the major striatal sensory motor function examined in animals not pre-injured. Connectivities included the cortical, nigral, and raphe to the striatal graft, and the striatal graft to the nigra, respectively. The Phaseolus oulgaris agglutinin leuca (PHAL) anterograde-tracing method is used to detect cortical input to the transplants, because the injection of PHAL into host cortex produces more consistent results than the injection of horseradish peroxidase (HRP) into transplants of unknown shape, size, and location. The immunocytochemical staining of serotonin (5-HT) and the catecholaminergic enzyme, tyrosine hydroxylase (TH), are used to detect the 5-HT-raphe and dopamine (DA)-nigral inputs to graft. HRP retrograde-tracing methods are used to detect the innervation of transplant into host nigra, because the HRP-injection into host nigra and labeling in the striatum and striatal graft are quite reproducible.

Original languageEnglish (US)
Pages (from-to)441-458
Number of pages18
JournalProgress in Brain Research
Volume82
Issue numberC
DOIs
StatePublished - Jan 1 1990

ASJC Scopus subject areas

  • Neuroscience(all)

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