Consensus guidelines for the use and interpretation of angiogenesis assays

Patrycja Nowak-Sliwinska, Kari Alitalo, Elizabeth Allen, Andrey Anisimov, Alfred C. Aplin, Robert Auerbach, Hellmut G. Augustin, David O. Bates, Judy R. van Beijnum, R. Hugh F. Bender, Gabriele Bergers, Andreas Bikfalvi, Joyce Bischoff, Barbara C. Böck, Peter C. Brooks, Federico Bussolino, Bertan Cakir, Peter Carmeliet, Daniel Castranova, Anca M. CimpeanOndine Cleaver, George Coukos, George E. Davis, Michele De Palma, Anna Dimberg, Ruud P.M. Dings, Valentin Djonov, Andrew C. Dudley, Neil P. Dufton, Sarah Maria Fendt, Napoleone Ferrara, Marcus Fruttiger, Dai Fukumura, Bart Ghesquière, Yan Gong, Robert J. Griffin, Adrian L. Harris, Christopher C.W. Hughes, Nan W. Hultgren, M. Luisa Iruela-Arispe, Melita Irving, Rakesh K. Jain, Raghu Kalluri, Joanna Kalucka, Robert S. Kerbel, Jan Kitajewski, Ingeborg Klaassen, Hynda K. Kleinmann, Pieter Koolwijk, Elisabeth Kuczynski, Brenda R. Kwak, Koen Marien, Juan M. Melero-Martin, Lance L. Munn, Roberto F. Nicosia, Agnes Noel, Jussi Nurro, Anna Karin Olsson, Tatiana V. Petrova, Kristian Pietras, Roberto Pili, Jeffrey W. Pollard, Mark J. Post, Paul H.A. Quax, Gabriel A. Rabinovich, Marius Raica, Anna M. Randi, Domenico Ribatti, Curzio Ruegg, Reinier O. Schlingemann, Stefan Schulte-Merker, Lois E.H. Smith, Jonathan W. Song, Steven A. Stacker, Jimmy Stalin, Amber N. Stratman, Maureen Van de Velde, Victor W.M. van Hinsbergh, Peter B. Vermeulen, Johannes Waltenberger, Brant M. Weinstein, Hong Xin, Bahar Yetkin-Arik, Seppo Yla-Herttuala, Mervin Yoder, Arjan W. Griffioen

Research output: Contribution to journalReview article

68 Citations (Scopus)

Abstract

The formation of new blood vessels, or angiogenesis, is a complex process that plays important roles in growth and development, tissue and organ regeneration, as well as numerous pathological conditions. Angiogenesis undergoes multiple discrete steps that can be individually evaluated and quantified by a large number of bioassays. These independent assessments hold advantages but also have limitations. This article describes in vivo, ex vivo, and in vitro bioassays that are available for the evaluation of angiogenesis and highlights critical aspects that are relevant for their execution and proper interpretation. As such, this collaborative work is the first edition of consensus guidelines on angiogenesis bioassays to serve for current and future reference.

Original languageEnglish (US)
Pages (from-to)425-532
Number of pages108
JournalAngiogenesis
Volume21
Issue number3
DOIs
StatePublished - Aug 1 2018

Fingerprint

Bioassay
Biological Assay
Assays
Guidelines
Blood vessels
Growth and Development
Blood Vessels
Regeneration
Tissue

Keywords

  • Angiogenesis
  • Aortic ring
  • Chorioallantoic membrane (CAM)
  • Corneal angiogenesis
  • Endothelial cell migration
  • Hindlimb ischemia
  • Intussusceptive angiogenesis
  • Microfluidic
  • Myocardial angiogenesis
  • Myocardial angiogenesis
  • Plug assay
  • Proliferation
  • Recombinant proteins
  • Retinal vasculature
  • Tip cells
  • Vascular network
  • Vessel co-option
  • Zebrafish

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cancer Research

Cite this

Nowak-Sliwinska, P., Alitalo, K., Allen, E., Anisimov, A., Aplin, A. C., Auerbach, R., ... Griffioen, A. W. (2018). Consensus guidelines for the use and interpretation of angiogenesis assays. Angiogenesis, 21(3), 425-532. https://doi.org/10.1007/s10456-018-9613-x

Consensus guidelines for the use and interpretation of angiogenesis assays. / Nowak-Sliwinska, Patrycja; Alitalo, Kari; Allen, Elizabeth; Anisimov, Andrey; Aplin, Alfred C.; Auerbach, Robert; Augustin, Hellmut G.; Bates, David O.; van Beijnum, Judy R.; Bender, R. Hugh F.; Bergers, Gabriele; Bikfalvi, Andreas; Bischoff, Joyce; Böck, Barbara C.; Brooks, Peter C.; Bussolino, Federico; Cakir, Bertan; Carmeliet, Peter; Castranova, Daniel; Cimpean, Anca M.; Cleaver, Ondine; Coukos, George; Davis, George E.; De Palma, Michele; Dimberg, Anna; Dings, Ruud P.M.; Djonov, Valentin; Dudley, Andrew C.; Dufton, Neil P.; Fendt, Sarah Maria; Ferrara, Napoleone; Fruttiger, Marcus; Fukumura, Dai; Ghesquière, Bart; Gong, Yan; Griffin, Robert J.; Harris, Adrian L.; Hughes, Christopher C.W.; Hultgren, Nan W.; Iruela-Arispe, M. Luisa; Irving, Melita; Jain, Rakesh K.; Kalluri, Raghu; Kalucka, Joanna; Kerbel, Robert S.; Kitajewski, Jan; Klaassen, Ingeborg; Kleinmann, Hynda K.; Koolwijk, Pieter; Kuczynski, Elisabeth; Kwak, Brenda R.; Marien, Koen; Melero-Martin, Juan M.; Munn, Lance L.; Nicosia, Roberto F.; Noel, Agnes; Nurro, Jussi; Olsson, Anna Karin; Petrova, Tatiana V.; Pietras, Kristian; Pili, Roberto; Pollard, Jeffrey W.; Post, Mark J.; Quax, Paul H.A.; Rabinovich, Gabriel A.; Raica, Marius; Randi, Anna M.; Ribatti, Domenico; Ruegg, Curzio; Schlingemann, Reinier O.; Schulte-Merker, Stefan; Smith, Lois E.H.; Song, Jonathan W.; Stacker, Steven A.; Stalin, Jimmy; Stratman, Amber N.; Van de Velde, Maureen; van Hinsbergh, Victor W.M.; Vermeulen, Peter B.; Waltenberger, Johannes; Weinstein, Brant M.; Xin, Hong; Yetkin-Arik, Bahar; Yla-Herttuala, Seppo; Yoder, Mervin; Griffioen, Arjan W.

In: Angiogenesis, Vol. 21, No. 3, 01.08.2018, p. 425-532.

Research output: Contribution to journalReview article

Nowak-Sliwinska, P, Alitalo, K, Allen, E, Anisimov, A, Aplin, AC, Auerbach, R, Augustin, HG, Bates, DO, van Beijnum, JR, Bender, RHF, Bergers, G, Bikfalvi, A, Bischoff, J, Böck, BC, Brooks, PC, Bussolino, F, Cakir, B, Carmeliet, P, Castranova, D, Cimpean, AM, Cleaver, O, Coukos, G, Davis, GE, De Palma, M, Dimberg, A, Dings, RPM, Djonov, V, Dudley, AC, Dufton, NP, Fendt, SM, Ferrara, N, Fruttiger, M, Fukumura, D, Ghesquière, B, Gong, Y, Griffin, RJ, Harris, AL, Hughes, CCW, Hultgren, NW, Iruela-Arispe, ML, Irving, M, Jain, RK, Kalluri, R, Kalucka, J, Kerbel, RS, Kitajewski, J, Klaassen, I, Kleinmann, HK, Koolwijk, P, Kuczynski, E, Kwak, BR, Marien, K, Melero-Martin, JM, Munn, LL, Nicosia, RF, Noel, A, Nurro, J, Olsson, AK, Petrova, TV, Pietras, K, Pili, R, Pollard, JW, Post, MJ, Quax, PHA, Rabinovich, GA, Raica, M, Randi, AM, Ribatti, D, Ruegg, C, Schlingemann, RO, Schulte-Merker, S, Smith, LEH, Song, JW, Stacker, SA, Stalin, J, Stratman, AN, Van de Velde, M, van Hinsbergh, VWM, Vermeulen, PB, Waltenberger, J, Weinstein, BM, Xin, H, Yetkin-Arik, B, Yla-Herttuala, S, Yoder, M & Griffioen, AW 2018, 'Consensus guidelines for the use and interpretation of angiogenesis assays', Angiogenesis, vol. 21, no. 3, pp. 425-532. https://doi.org/10.1007/s10456-018-9613-x
Nowak-Sliwinska P, Alitalo K, Allen E, Anisimov A, Aplin AC, Auerbach R et al. Consensus guidelines for the use and interpretation of angiogenesis assays. Angiogenesis. 2018 Aug 1;21(3):425-532. https://doi.org/10.1007/s10456-018-9613-x
Nowak-Sliwinska, Patrycja ; Alitalo, Kari ; Allen, Elizabeth ; Anisimov, Andrey ; Aplin, Alfred C. ; Auerbach, Robert ; Augustin, Hellmut G. ; Bates, David O. ; van Beijnum, Judy R. ; Bender, R. Hugh F. ; Bergers, Gabriele ; Bikfalvi, Andreas ; Bischoff, Joyce ; Böck, Barbara C. ; Brooks, Peter C. ; Bussolino, Federico ; Cakir, Bertan ; Carmeliet, Peter ; Castranova, Daniel ; Cimpean, Anca M. ; Cleaver, Ondine ; Coukos, George ; Davis, George E. ; De Palma, Michele ; Dimberg, Anna ; Dings, Ruud P.M. ; Djonov, Valentin ; Dudley, Andrew C. ; Dufton, Neil P. ; Fendt, Sarah Maria ; Ferrara, Napoleone ; Fruttiger, Marcus ; Fukumura, Dai ; Ghesquière, Bart ; Gong, Yan ; Griffin, Robert J. ; Harris, Adrian L. ; Hughes, Christopher C.W. ; Hultgren, Nan W. ; Iruela-Arispe, M. Luisa ; Irving, Melita ; Jain, Rakesh K. ; Kalluri, Raghu ; Kalucka, Joanna ; Kerbel, Robert S. ; Kitajewski, Jan ; Klaassen, Ingeborg ; Kleinmann, Hynda K. ; Koolwijk, Pieter ; Kuczynski, Elisabeth ; Kwak, Brenda R. ; Marien, Koen ; Melero-Martin, Juan M. ; Munn, Lance L. ; Nicosia, Roberto F. ; Noel, Agnes ; Nurro, Jussi ; Olsson, Anna Karin ; Petrova, Tatiana V. ; Pietras, Kristian ; Pili, Roberto ; Pollard, Jeffrey W. ; Post, Mark J. ; Quax, Paul H.A. ; Rabinovich, Gabriel A. ; Raica, Marius ; Randi, Anna M. ; Ribatti, Domenico ; Ruegg, Curzio ; Schlingemann, Reinier O. ; Schulte-Merker, Stefan ; Smith, Lois E.H. ; Song, Jonathan W. ; Stacker, Steven A. ; Stalin, Jimmy ; Stratman, Amber N. ; Van de Velde, Maureen ; van Hinsbergh, Victor W.M. ; Vermeulen, Peter B. ; Waltenberger, Johannes ; Weinstein, Brant M. ; Xin, Hong ; Yetkin-Arik, Bahar ; Yla-Herttuala, Seppo ; Yoder, Mervin ; Griffioen, Arjan W. / Consensus guidelines for the use and interpretation of angiogenesis assays. In: Angiogenesis. 2018 ; Vol. 21, No. 3. pp. 425-532.
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T1 - Consensus guidelines for the use and interpretation of angiogenesis assays

AU - Nowak-Sliwinska, Patrycja

AU - Alitalo, Kari

AU - Allen, Elizabeth

AU - Anisimov, Andrey

AU - Aplin, Alfred C.

AU - Auerbach, Robert

AU - Augustin, Hellmut G.

AU - Bates, David O.

AU - van Beijnum, Judy R.

AU - Bender, R. Hugh F.

AU - Bergers, Gabriele

AU - Bikfalvi, Andreas

AU - Bischoff, Joyce

AU - Böck, Barbara C.

AU - Brooks, Peter C.

AU - Bussolino, Federico

AU - Cakir, Bertan

AU - Carmeliet, Peter

AU - Castranova, Daniel

AU - Cimpean, Anca M.

AU - Cleaver, Ondine

AU - Coukos, George

AU - Davis, George E.

AU - De Palma, Michele

AU - Dimberg, Anna

AU - Dings, Ruud P.M.

AU - Djonov, Valentin

AU - Dudley, Andrew C.

AU - Dufton, Neil P.

AU - Fendt, Sarah Maria

AU - Ferrara, Napoleone

AU - Fruttiger, Marcus

AU - Fukumura, Dai

AU - Ghesquière, Bart

AU - Gong, Yan

AU - Griffin, Robert J.

AU - Harris, Adrian L.

AU - Hughes, Christopher C.W.

AU - Hultgren, Nan W.

AU - Iruela-Arispe, M. Luisa

AU - Irving, Melita

AU - Jain, Rakesh K.

AU - Kalluri, Raghu

AU - Kalucka, Joanna

AU - Kerbel, Robert S.

AU - Kitajewski, Jan

AU - Klaassen, Ingeborg

AU - Kleinmann, Hynda K.

AU - Koolwijk, Pieter

AU - Kuczynski, Elisabeth

AU - Kwak, Brenda R.

AU - Marien, Koen

AU - Melero-Martin, Juan M.

AU - Munn, Lance L.

AU - Nicosia, Roberto F.

AU - Noel, Agnes

AU - Nurro, Jussi

AU - Olsson, Anna Karin

AU - Petrova, Tatiana V.

AU - Pietras, Kristian

AU - Pili, Roberto

AU - Pollard, Jeffrey W.

AU - Post, Mark J.

AU - Quax, Paul H.A.

AU - Rabinovich, Gabriel A.

AU - Raica, Marius

AU - Randi, Anna M.

AU - Ribatti, Domenico

AU - Ruegg, Curzio

AU - Schlingemann, Reinier O.

AU - Schulte-Merker, Stefan

AU - Smith, Lois E.H.

AU - Song, Jonathan W.

AU - Stacker, Steven A.

AU - Stalin, Jimmy

AU - Stratman, Amber N.

AU - Van de Velde, Maureen

AU - van Hinsbergh, Victor W.M.

AU - Vermeulen, Peter B.

AU - Waltenberger, Johannes

AU - Weinstein, Brant M.

AU - Xin, Hong

AU - Yetkin-Arik, Bahar

AU - Yla-Herttuala, Seppo

AU - Yoder, Mervin

AU - Griffioen, Arjan W.

PY - 2018/8/1

Y1 - 2018/8/1

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AB - The formation of new blood vessels, or angiogenesis, is a complex process that plays important roles in growth and development, tissue and organ regeneration, as well as numerous pathological conditions. Angiogenesis undergoes multiple discrete steps that can be individually evaluated and quantified by a large number of bioassays. These independent assessments hold advantages but also have limitations. This article describes in vivo, ex vivo, and in vitro bioassays that are available for the evaluation of angiogenesis and highlights critical aspects that are relevant for their execution and proper interpretation. As such, this collaborative work is the first edition of consensus guidelines on angiogenesis bioassays to serve for current and future reference.

KW - Angiogenesis

KW - Aortic ring

KW - Chorioallantoic membrane (CAM)

KW - Corneal angiogenesis

KW - Endothelial cell migration

KW - Hindlimb ischemia

KW - Intussusceptive angiogenesis

KW - Microfluidic

KW - Myocardial angiogenesis

KW - Myocardial angiogenesis

KW - Plug assay

KW - Proliferation

KW - Recombinant proteins

KW - Retinal vasculature

KW - Tip cells

KW - Vascular network

KW - Vessel co-option

KW - Zebrafish

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U2 - 10.1007/s10456-018-9613-x

DO - 10.1007/s10456-018-9613-x

M3 - Review article

AN - SCOPUS:85046903965

VL - 21

SP - 425

EP - 532

JO - Angiogenesis

JF - Angiogenesis

SN - 0969-6970

IS - 3

ER -