Conserved genetic findings in metastatic bladder cancer: A possible utility of allelic loss of chromosomes 9p21 and 17p13 in diagnosis

Liang Cheng, D. G. Bostwick, G. Li, Shaobo Zhang, Alexander Vortmeyer, Z. Zhuang

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Context. - Molecular analysis of microsatellite alterations of biologically distinct tumor cell subpopulations from the same patient may aid in the determination of tumor origin and further our understanding of the genetic basis of cancer progression. Design. - The authors examined the pattern of allelic loss with polymorphic microsatellite markers on chromosome 9p21 (D9S161, D9S171, IFNA), regions of putative tumor suppressor gene p16, and on chromosome 17p13 (TP53), the p53 locus, in matched primary and metastatic bladder cancers from 9 patients. All patients underwent cystectomy for bladder cancer and had regional lymph node metastases at the time of surgery. Genomic DNA was prepared from primary cancers and matched synchronous lymph node metastases using a microdissection method. Results. - The overall frequency of allelic loss was 78% in primary cancer and 89% in paired metastatic cancer. The frequency of allelic loss in the primary cancer was 86% with D9S161, 67% with D9S171, 71% with IFNA, and 80% with TP53. The frequency of allelic loss in matched metastatic cancer was 100% with D9S161, 62% with D9S171, 71% with IFNA, and 80% with TP53. An identical pattern of allelic imbalance (allelic loss or retention) at multiple DNA loci was observed in matched primary and metastatic carcinoma in 8 (88%) cases. One case showed allelic loss in the metastasis, but not in the primary cancer. Conclusions. - The pattern of allelic loss at chromosome 9p21 (p16) and 17p13 (p53) was generally maintained during cancer progression to metastasis, and identical allelic loss in primary cancer was conserved in paired metastatic carcinoma. These data suggest that these genetic changes may be useful in establishing a diagnosis and determining tumor origins in difficult cases.

Original languageEnglish
Pages (from-to)1197-1199
Number of pages3
JournalArchives of Pathology and Laboratory Medicine
Volume125
Issue number9
StatePublished - 2001

Fingerprint

Loss of Heterozygosity
Urinary Bladder Neoplasms
Chromosomes
Neoplasms
Neoplasm Metastasis
Microsatellite Repeats
Lymph Nodes
Allelic Imbalance
Carcinoma
Microdissection
Cystectomy
DNA
Tumor Suppressor Genes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medical Laboratory Technology

Cite this

@article{b31f830130804855aee7c54d0f886623,
title = "Conserved genetic findings in metastatic bladder cancer: A possible utility of allelic loss of chromosomes 9p21 and 17p13 in diagnosis",
abstract = "Context. - Molecular analysis of microsatellite alterations of biologically distinct tumor cell subpopulations from the same patient may aid in the determination of tumor origin and further our understanding of the genetic basis of cancer progression. Design. - The authors examined the pattern of allelic loss with polymorphic microsatellite markers on chromosome 9p21 (D9S161, D9S171, IFNA), regions of putative tumor suppressor gene p16, and on chromosome 17p13 (TP53), the p53 locus, in matched primary and metastatic bladder cancers from 9 patients. All patients underwent cystectomy for bladder cancer and had regional lymph node metastases at the time of surgery. Genomic DNA was prepared from primary cancers and matched synchronous lymph node metastases using a microdissection method. Results. - The overall frequency of allelic loss was 78{\%} in primary cancer and 89{\%} in paired metastatic cancer. The frequency of allelic loss in the primary cancer was 86{\%} with D9S161, 67{\%} with D9S171, 71{\%} with IFNA, and 80{\%} with TP53. The frequency of allelic loss in matched metastatic cancer was 100{\%} with D9S161, 62{\%} with D9S171, 71{\%} with IFNA, and 80{\%} with TP53. An identical pattern of allelic imbalance (allelic loss or retention) at multiple DNA loci was observed in matched primary and metastatic carcinoma in 8 (88{\%}) cases. One case showed allelic loss in the metastasis, but not in the primary cancer. Conclusions. - The pattern of allelic loss at chromosome 9p21 (p16) and 17p13 (p53) was generally maintained during cancer progression to metastasis, and identical allelic loss in primary cancer was conserved in paired metastatic carcinoma. These data suggest that these genetic changes may be useful in establishing a diagnosis and determining tumor origins in difficult cases.",
author = "Liang Cheng and Bostwick, {D. G.} and G. Li and Shaobo Zhang and Alexander Vortmeyer and Z. Zhuang",
year = "2001",
language = "English",
volume = "125",
pages = "1197--1199",
journal = "Archives of Pathology and Laboratory Medicine",
issn = "0003-9985",
publisher = "College of American Pathologists",
number = "9",

}

TY - JOUR

T1 - Conserved genetic findings in metastatic bladder cancer

T2 - A possible utility of allelic loss of chromosomes 9p21 and 17p13 in diagnosis

AU - Cheng, Liang

AU - Bostwick, D. G.

AU - Li, G.

AU - Zhang, Shaobo

AU - Vortmeyer, Alexander

AU - Zhuang, Z.

PY - 2001

Y1 - 2001

N2 - Context. - Molecular analysis of microsatellite alterations of biologically distinct tumor cell subpopulations from the same patient may aid in the determination of tumor origin and further our understanding of the genetic basis of cancer progression. Design. - The authors examined the pattern of allelic loss with polymorphic microsatellite markers on chromosome 9p21 (D9S161, D9S171, IFNA), regions of putative tumor suppressor gene p16, and on chromosome 17p13 (TP53), the p53 locus, in matched primary and metastatic bladder cancers from 9 patients. All patients underwent cystectomy for bladder cancer and had regional lymph node metastases at the time of surgery. Genomic DNA was prepared from primary cancers and matched synchronous lymph node metastases using a microdissection method. Results. - The overall frequency of allelic loss was 78% in primary cancer and 89% in paired metastatic cancer. The frequency of allelic loss in the primary cancer was 86% with D9S161, 67% with D9S171, 71% with IFNA, and 80% with TP53. The frequency of allelic loss in matched metastatic cancer was 100% with D9S161, 62% with D9S171, 71% with IFNA, and 80% with TP53. An identical pattern of allelic imbalance (allelic loss or retention) at multiple DNA loci was observed in matched primary and metastatic carcinoma in 8 (88%) cases. One case showed allelic loss in the metastasis, but not in the primary cancer. Conclusions. - The pattern of allelic loss at chromosome 9p21 (p16) and 17p13 (p53) was generally maintained during cancer progression to metastasis, and identical allelic loss in primary cancer was conserved in paired metastatic carcinoma. These data suggest that these genetic changes may be useful in establishing a diagnosis and determining tumor origins in difficult cases.

AB - Context. - Molecular analysis of microsatellite alterations of biologically distinct tumor cell subpopulations from the same patient may aid in the determination of tumor origin and further our understanding of the genetic basis of cancer progression. Design. - The authors examined the pattern of allelic loss with polymorphic microsatellite markers on chromosome 9p21 (D9S161, D9S171, IFNA), regions of putative tumor suppressor gene p16, and on chromosome 17p13 (TP53), the p53 locus, in matched primary and metastatic bladder cancers from 9 patients. All patients underwent cystectomy for bladder cancer and had regional lymph node metastases at the time of surgery. Genomic DNA was prepared from primary cancers and matched synchronous lymph node metastases using a microdissection method. Results. - The overall frequency of allelic loss was 78% in primary cancer and 89% in paired metastatic cancer. The frequency of allelic loss in the primary cancer was 86% with D9S161, 67% with D9S171, 71% with IFNA, and 80% with TP53. The frequency of allelic loss in matched metastatic cancer was 100% with D9S161, 62% with D9S171, 71% with IFNA, and 80% with TP53. An identical pattern of allelic imbalance (allelic loss or retention) at multiple DNA loci was observed in matched primary and metastatic carcinoma in 8 (88%) cases. One case showed allelic loss in the metastasis, but not in the primary cancer. Conclusions. - The pattern of allelic loss at chromosome 9p21 (p16) and 17p13 (p53) was generally maintained during cancer progression to metastasis, and identical allelic loss in primary cancer was conserved in paired metastatic carcinoma. These data suggest that these genetic changes may be useful in establishing a diagnosis and determining tumor origins in difficult cases.

UR - http://www.scopus.com/inward/record.url?scp=0034855014&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034855014&partnerID=8YFLogxK

M3 - Article

C2 - 11520271

AN - SCOPUS:0034855014

VL - 125

SP - 1197

EP - 1199

JO - Archives of Pathology and Laboratory Medicine

JF - Archives of Pathology and Laboratory Medicine

SN - 0003-9985

IS - 9

ER -