Constitutive expression of LY-6.A2 on murine keratinocytes and inducible expression on TCRγδ+ dendritic epidermal T cells

Harm HogenEsch, Bernard de Geus, Frans Tielen, Jan Rozing

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

We investigated the expression of Ly-6.A2 on isolated murine epidermal cells by flow cytometry. Ly-6.A2 was expressed on 61% of keratinocytes and 6% of dendritic epidermal T cells of C57BL mice. Phosphatidylinositol-specific phospholipase C removed Ly-6.A2, indicating that the antigen is anchored to the keratinocyte membrane via a glycosyl-phosphatidylinositol anchor similar to its attachment to the membrane of lymphocytes. Induction of dermatitis by topical application of PMA increased the expression of Ly-6.A2 of TCRγδ+ dendritic epidermal T cells and did not change its expression on keratinocytes. The increased expression of Ly-6.A2 on dendritic epidermal T cells was transient and reached a peak at 4 days after application of PMA, when 55% of the cells were positive.

Original languageEnglish (US)
Pages (from-to)114-121
Number of pages8
JournalJournal of Dermatological Science
Volume5
Issue number2
DOIs
StatePublished - Apr 1993

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Keywords

  • Epidermis
  • Glycosyl-phosphatidylinositol
  • Keratinocytes
  • Ly-6
  • Thy-1

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology

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