Construction of a couple of eukaryotic expression recombinants containing the gene fragment of 42kD C-terminal region of Plasmodium falciparum merozoite surface protein 1

Jun Miao, Caifang Xue, Andy Yu

Research output: Contribution to journalArticle

Abstract

Objectives: Nucleic acid vaccine has several major advantages over other types of available vaccines, such as in situ eukaryotic expression and generation of extensively cellular response. To construct a couple of eukaryotic expression recombinants containing the gene fragment of C-terminal region (coding for 42kD protein) of Plasmodium falciparum(P.f)merozoite surface protein 1(MSP1), which could be a candidate nucleic acid vaccine. Methods: The gene fragment of C-terminal region (coding for 42kD protein) of MSP1 from P.f FUP strain was cloned into eukaryotic expression plasmid VR1012 for intracellular expression and VR1012/TPA for secretion by commonly used molecular clone methods. The eukaryotic expression recombinants were identified by PCR and restriction enzyme. Results: The recombinants VR1012/MSP1-42 and VR1012/TPA/MSP1-42 were successfully constructed. Conclusions: Up to now only nucleic acid vaccine of rodent malaria was reported. This study provides a good basis for us to find out an effective P.f erythrocytic stage nucleic acid vaccine . The protective immune response to these vaccines will be studied in the future.

Original languageEnglish (US)
Pages (from-to)186-188
Number of pages3
JournalChinese Journal of Microbiology and Immunology
Volume18
Issue number3
StatePublished - May 1998
Externally publishedYes

Fingerprint

Merozoite Surface Protein 1
DNA Vaccines
Plasmodium falciparum
Open Reading Frames
Genes
Vaccines
Malaria
Rodentia
Plasmids
Clone Cells
Polymerase Chain Reaction
Enzymes

Keywords

  • Gene expression
  • Merozoite surface protein 1
  • Nucleic acid vaccine
  • Plasmid
  • Plasmodium falciparum

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology (medical)

Cite this

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title = "Construction of a couple of eukaryotic expression recombinants containing the gene fragment of 42kD C-terminal region of Plasmodium falciparum merozoite surface protein 1",
abstract = "Objectives: Nucleic acid vaccine has several major advantages over other types of available vaccines, such as in situ eukaryotic expression and generation of extensively cellular response. To construct a couple of eukaryotic expression recombinants containing the gene fragment of C-terminal region (coding for 42kD protein) of Plasmodium falciparum(P.f)merozoite surface protein 1(MSP1), which could be a candidate nucleic acid vaccine. Methods: The gene fragment of C-terminal region (coding for 42kD protein) of MSP1 from P.f FUP strain was cloned into eukaryotic expression plasmid VR1012 for intracellular expression and VR1012/TPA for secretion by commonly used molecular clone methods. The eukaryotic expression recombinants were identified by PCR and restriction enzyme. Results: The recombinants VR1012/MSP1-42 and VR1012/TPA/MSP1-42 were successfully constructed. Conclusions: Up to now only nucleic acid vaccine of rodent malaria was reported. This study provides a good basis for us to find out an effective P.f erythrocytic stage nucleic acid vaccine . The protective immune response to these vaccines will be studied in the future.",
keywords = "Gene expression, Merozoite surface protein 1, Nucleic acid vaccine, Plasmid, Plasmodium falciparum",
author = "Jun Miao and Caifang Xue and Andy Yu",
year = "1998",
month = "5",
language = "English (US)",
volume = "18",
pages = "186--188",
journal = "Chinese Journal of Microbiology and Immunology",
issn = "0254-5101",
publisher = "Society of Microbiology and Immunology",
number = "3",

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TY - JOUR

T1 - Construction of a couple of eukaryotic expression recombinants containing the gene fragment of 42kD C-terminal region of Plasmodium falciparum merozoite surface protein 1

AU - Miao, Jun

AU - Xue, Caifang

AU - Yu, Andy

PY - 1998/5

Y1 - 1998/5

N2 - Objectives: Nucleic acid vaccine has several major advantages over other types of available vaccines, such as in situ eukaryotic expression and generation of extensively cellular response. To construct a couple of eukaryotic expression recombinants containing the gene fragment of C-terminal region (coding for 42kD protein) of Plasmodium falciparum(P.f)merozoite surface protein 1(MSP1), which could be a candidate nucleic acid vaccine. Methods: The gene fragment of C-terminal region (coding for 42kD protein) of MSP1 from P.f FUP strain was cloned into eukaryotic expression plasmid VR1012 for intracellular expression and VR1012/TPA for secretion by commonly used molecular clone methods. The eukaryotic expression recombinants were identified by PCR and restriction enzyme. Results: The recombinants VR1012/MSP1-42 and VR1012/TPA/MSP1-42 were successfully constructed. Conclusions: Up to now only nucleic acid vaccine of rodent malaria was reported. This study provides a good basis for us to find out an effective P.f erythrocytic stage nucleic acid vaccine . The protective immune response to these vaccines will be studied in the future.

AB - Objectives: Nucleic acid vaccine has several major advantages over other types of available vaccines, such as in situ eukaryotic expression and generation of extensively cellular response. To construct a couple of eukaryotic expression recombinants containing the gene fragment of C-terminal region (coding for 42kD protein) of Plasmodium falciparum(P.f)merozoite surface protein 1(MSP1), which could be a candidate nucleic acid vaccine. Methods: The gene fragment of C-terminal region (coding for 42kD protein) of MSP1 from P.f FUP strain was cloned into eukaryotic expression plasmid VR1012 for intracellular expression and VR1012/TPA for secretion by commonly used molecular clone methods. The eukaryotic expression recombinants were identified by PCR and restriction enzyme. Results: The recombinants VR1012/MSP1-42 and VR1012/TPA/MSP1-42 were successfully constructed. Conclusions: Up to now only nucleic acid vaccine of rodent malaria was reported. This study provides a good basis for us to find out an effective P.f erythrocytic stage nucleic acid vaccine . The protective immune response to these vaccines will be studied in the future.

KW - Gene expression

KW - Merozoite surface protein 1

KW - Nucleic acid vaccine

KW - Plasmid

KW - Plasmodium falciparum

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