Construction of bivalent vaccine candidate expressing CS3 fimbriae and cholera toxin B subunit

Zizheng Dong, Shuqin Li, Zhaoshan Zhang

Research output: Contribution to journalArticle

Abstract

Enterotoxigenic Escherichia coli (ETEC) is one of the most important pathogens of diarrhea in children under age 5 years and in travelers. A multicomponent vaccine made up of anti-colonization factor immunity plus toxoids may provide the best immune stimulation. A recombinant strain which could express bivalent antigens of CS3 fimbriae and cholera toxin B subunit (CT-B) was constructed using plasmid vector bluescript. Inoculating mice orally and immunizing rabbits intramuscularly demonstrated that the recombinant strain could induce immune response against the two antigens.

Original languageEnglish (US)
Pages (from-to)348-351
Number of pages4
JournalChinese Journal of Microbiology and Immunology
Volume17
Issue number5
StatePublished - Sep 1997
Externally publishedYes

Fingerprint

Cholera Toxin
Vaccines
Toxoids
Enterotoxigenic Escherichia coli
Antigens
Diarrhea
Immunity
Plasmids
Rabbits

Keywords

  • CS3 fimbriae
  • CT-B
  • Escherichia coli
  • Genetic vaccine

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology (medical)

Cite this

Construction of bivalent vaccine candidate expressing CS3 fimbriae and cholera toxin B subunit. / Dong, Zizheng; Li, Shuqin; Zhang, Zhaoshan.

In: Chinese Journal of Microbiology and Immunology, Vol. 17, No. 5, 09.1997, p. 348-351.

Research output: Contribution to journalArticle

@article{4b5b83492e4a44afafe14a31b5d5faec,
title = "Construction of bivalent vaccine candidate expressing CS3 fimbriae and cholera toxin B subunit",
abstract = "Enterotoxigenic Escherichia coli (ETEC) is one of the most important pathogens of diarrhea in children under age 5 years and in travelers. A multicomponent vaccine made up of anti-colonization factor immunity plus toxoids may provide the best immune stimulation. A recombinant strain which could express bivalent antigens of CS3 fimbriae and cholera toxin B subunit (CT-B) was constructed using plasmid vector bluescript. Inoculating mice orally and immunizing rabbits intramuscularly demonstrated that the recombinant strain could induce immune response against the two antigens.",
keywords = "CS3 fimbriae, CT-B, Escherichia coli, Genetic vaccine",
author = "Zizheng Dong and Shuqin Li and Zhaoshan Zhang",
year = "1997",
month = "9",
language = "English (US)",
volume = "17",
pages = "348--351",
journal = "Chinese Journal of Microbiology and Immunology",
issn = "0254-5101",
publisher = "Society of Microbiology and Immunology",
number = "5",

}

TY - JOUR

T1 - Construction of bivalent vaccine candidate expressing CS3 fimbriae and cholera toxin B subunit

AU - Dong, Zizheng

AU - Li, Shuqin

AU - Zhang, Zhaoshan

PY - 1997/9

Y1 - 1997/9

N2 - Enterotoxigenic Escherichia coli (ETEC) is one of the most important pathogens of diarrhea in children under age 5 years and in travelers. A multicomponent vaccine made up of anti-colonization factor immunity plus toxoids may provide the best immune stimulation. A recombinant strain which could express bivalent antigens of CS3 fimbriae and cholera toxin B subunit (CT-B) was constructed using plasmid vector bluescript. Inoculating mice orally and immunizing rabbits intramuscularly demonstrated that the recombinant strain could induce immune response against the two antigens.

AB - Enterotoxigenic Escherichia coli (ETEC) is one of the most important pathogens of diarrhea in children under age 5 years and in travelers. A multicomponent vaccine made up of anti-colonization factor immunity plus toxoids may provide the best immune stimulation. A recombinant strain which could express bivalent antigens of CS3 fimbriae and cholera toxin B subunit (CT-B) was constructed using plasmid vector bluescript. Inoculating mice orally and immunizing rabbits intramuscularly demonstrated that the recombinant strain could induce immune response against the two antigens.

KW - CS3 fimbriae

KW - CT-B

KW - Escherichia coli

KW - Genetic vaccine

UR - http://www.scopus.com/inward/record.url?scp=0343554081&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0343554081&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0343554081

VL - 17

SP - 348

EP - 351

JO - Chinese Journal of Microbiology and Immunology

JF - Chinese Journal of Microbiology and Immunology

SN - 0254-5101

IS - 5

ER -