Construction of CyclinD (-/-)/patched (+/-) double knockout mice

Shi Jie Xin, Xuan Li, Molly Scheel, Wei Du

Research output: Contribution to journalArticle

Abstract

Objective: To construct CyclinD (CycD) (-/-)/Patched (Ptc) (+/-) double knockout mice to study the relationship between Hedgehog and Rb signaling pathways. Methods: Amphimixis was performed between female heterozygote CycD knockout mice CycD (+/-) and male heterozygote Ptc knockout mice Ptc (+/ -), thus CycD (+/-)/Ptc (+/-) double knockout mice were obtained. These mice underwent secondary matched and thus construct CycD (-/-)/Ptc (+/-) double knockout mice. Results: CycD (-/-)/Ptc (+/-) double knockout mice, CycD (+/-)/Ptc (+/-) heterozygote double knockout mice, and CycD knockout mice CycD (-/-)/Ptc (+/+) were gained. Conclusion: The method of amphimixis can construct CycD (-/-)/Ptc (+/-) double knockout mice that can be used to disclose the regulation between Ptc gene and CycD gene and the relativity between Hedgehog and Rb pathways in mammals.

Original languageEnglish (US)
Pages (from-to)185-188
Number of pages4
JournalZhonghua yi xue za zhi
Volume88
Issue number3
StatePublished - Jan 15 2008
Externally publishedYes

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Knockout Mice
Heterozygote
Hedgehogs
Genes
Mammals

Keywords

  • Cyclin D
  • Knockout mice
  • Patched

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Construction of CyclinD (-/-)/patched (+/-) double knockout mice. / Xin, Shi Jie; Li, Xuan; Scheel, Molly; Du, Wei.

In: Zhonghua yi xue za zhi, Vol. 88, No. 3, 15.01.2008, p. 185-188.

Research output: Contribution to journalArticle

Xin, Shi Jie ; Li, Xuan ; Scheel, Molly ; Du, Wei. / Construction of CyclinD (-/-)/patched (+/-) double knockout mice. In: Zhonghua yi xue za zhi. 2008 ; Vol. 88, No. 3. pp. 185-188.
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abstract = "Objective: To construct CyclinD (CycD) (-/-)/Patched (Ptc) (+/-) double knockout mice to study the relationship between Hedgehog and Rb signaling pathways. Methods: Amphimixis was performed between female heterozygote CycD knockout mice CycD (+/-) and male heterozygote Ptc knockout mice Ptc (+/ -), thus CycD (+/-)/Ptc (+/-) double knockout mice were obtained. These mice underwent secondary matched and thus construct CycD (-/-)/Ptc (+/-) double knockout mice. Results: CycD (-/-)/Ptc (+/-) double knockout mice, CycD (+/-)/Ptc (+/-) heterozygote double knockout mice, and CycD knockout mice CycD (-/-)/Ptc (+/+) were gained. Conclusion: The method of amphimixis can construct CycD (-/-)/Ptc (+/-) double knockout mice that can be used to disclose the regulation between Ptc gene and CycD gene and the relativity between Hedgehog and Rb pathways in mammals.",
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AU - Du, Wei

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N2 - Objective: To construct CyclinD (CycD) (-/-)/Patched (Ptc) (+/-) double knockout mice to study the relationship between Hedgehog and Rb signaling pathways. Methods: Amphimixis was performed between female heterozygote CycD knockout mice CycD (+/-) and male heterozygote Ptc knockout mice Ptc (+/ -), thus CycD (+/-)/Ptc (+/-) double knockout mice were obtained. These mice underwent secondary matched and thus construct CycD (-/-)/Ptc (+/-) double knockout mice. Results: CycD (-/-)/Ptc (+/-) double knockout mice, CycD (+/-)/Ptc (+/-) heterozygote double knockout mice, and CycD knockout mice CycD (-/-)/Ptc (+/+) were gained. Conclusion: The method of amphimixis can construct CycD (-/-)/Ptc (+/-) double knockout mice that can be used to disclose the regulation between Ptc gene and CycD gene and the relativity between Hedgehog and Rb pathways in mammals.

AB - Objective: To construct CyclinD (CycD) (-/-)/Patched (Ptc) (+/-) double knockout mice to study the relationship between Hedgehog and Rb signaling pathways. Methods: Amphimixis was performed between female heterozygote CycD knockout mice CycD (+/-) and male heterozygote Ptc knockout mice Ptc (+/ -), thus CycD (+/-)/Ptc (+/-) double knockout mice were obtained. These mice underwent secondary matched and thus construct CycD (-/-)/Ptc (+/-) double knockout mice. Results: CycD (-/-)/Ptc (+/-) double knockout mice, CycD (+/-)/Ptc (+/-) heterozygote double knockout mice, and CycD knockout mice CycD (-/-)/Ptc (+/+) were gained. Conclusion: The method of amphimixis can construct CycD (-/-)/Ptc (+/-) double knockout mice that can be used to disclose the regulation between Ptc gene and CycD gene and the relativity between Hedgehog and Rb pathways in mammals.

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