Construction of CyclinD (-/-)/patched (+/-) double knockout mice

Shi Jie Xin, Xuan Li, Molly Duman-Schell, Wei Du

Research output: Contribution to journalArticle


Objective: To construct CyclinD (CycD) (-/-)/Patched (Ptc) (+/-) double knockout mice to study the relationship between Hedgehog and Rb signaling pathways. Methods: Amphimixis was performed between female heterozygote CycD knockout mice CycD (+/-) and male heterozygote Ptc knockout mice Ptc (+/ -), thus CycD (+/-)/Ptc (+/-) double knockout mice were obtained. These mice underwent secondary matched and thus construct CycD (-/-)/Ptc (+/-) double knockout mice. Results: CycD (-/-)/Ptc (+/-) double knockout mice, CycD (+/-)/Ptc (+/-) heterozygote double knockout mice, and CycD knockout mice CycD (-/-)/Ptc (+/+) were gained. Conclusion: The method of amphimixis can construct CycD (-/-)/Ptc (+/-) double knockout mice that can be used to disclose the regulation between Ptc gene and CycD gene and the relativity between Hedgehog and Rb pathways in mammals.

Original languageEnglish (US)
Pages (from-to)185-188
Number of pages4
JournalNational Medical Journal of China
Issue number3
StatePublished - Jan 15 2008
Externally publishedYes


  • Cyclin D
  • Knockout mice
  • Patched

ASJC Scopus subject areas

  • Medicine(all)

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