Construction of YAC-based mammalian artificial chromosomes

Masashi Ikeno, Brenda Grimes, Tuneko Okazaki, Megumi Nakano, Kaori Saitoh, Harumi Hoshino, Niolette I. McGill, Howard Cooke, Hiroshi Masumoto

Research output: Contribution to journalArticle

317 Citations (Scopus)

Abstract

To construct a mammalian artificial chromosome (MAC), telomere repeats and selectable markers were introduced into a 100 kb yeast artificial chromosome (YAC) containing human centromeric DNA. This YAC, which has a regular repeat structure of alpha-satellite DNA and centromere protein B (CENP-B) boxes, efficiently formed MACs that segregated accurately and bound CENP-B, CENP-C, and CENP-E. The MACs appear to be about 1-5 Mb in size and contain YAC multimers. Structural analyses suggest that the MACs have not acquired host sequences and were formed by a de novo mechanism. The accurate segregation of the MACs suggests they have potential as vectors for introducing genes into mammals.

Original languageEnglish (US)
Pages (from-to)431-439
Number of pages9
JournalNature Biotechnology
Volume16
Issue number5
StatePublished - May 1998
Externally publishedYes

Fingerprint

Mammalian Artificial Chromosomes
Yeast Artificial Chromosomes
Centromere Protein B
Chromosomes
Yeast
Satellite DNA
DNA
Telomere
Proteins
Mammals
Genes
Satellites

Keywords

  • Alpha-satellite DNA
  • CENP-B boxes

ASJC Scopus subject areas

  • Microbiology

Cite this

Ikeno, M., Grimes, B., Okazaki, T., Nakano, M., Saitoh, K., Hoshino, H., ... Masumoto, H. (1998). Construction of YAC-based mammalian artificial chromosomes. Nature Biotechnology, 16(5), 431-439.

Construction of YAC-based mammalian artificial chromosomes. / Ikeno, Masashi; Grimes, Brenda; Okazaki, Tuneko; Nakano, Megumi; Saitoh, Kaori; Hoshino, Harumi; McGill, Niolette I.; Cooke, Howard; Masumoto, Hiroshi.

In: Nature Biotechnology, Vol. 16, No. 5, 05.1998, p. 431-439.

Research output: Contribution to journalArticle

Ikeno, M, Grimes, B, Okazaki, T, Nakano, M, Saitoh, K, Hoshino, H, McGill, NI, Cooke, H & Masumoto, H 1998, 'Construction of YAC-based mammalian artificial chromosomes', Nature Biotechnology, vol. 16, no. 5, pp. 431-439.
Ikeno M, Grimes B, Okazaki T, Nakano M, Saitoh K, Hoshino H et al. Construction of YAC-based mammalian artificial chromosomes. Nature Biotechnology. 1998 May;16(5):431-439.
Ikeno, Masashi ; Grimes, Brenda ; Okazaki, Tuneko ; Nakano, Megumi ; Saitoh, Kaori ; Hoshino, Harumi ; McGill, Niolette I. ; Cooke, Howard ; Masumoto, Hiroshi. / Construction of YAC-based mammalian artificial chromosomes. In: Nature Biotechnology. 1998 ; Vol. 16, No. 5. pp. 431-439.
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