Construction of YAC-based mammalian artificial chromosomes

Masashi Ikeno, Brenda Grimes, Tuneko Okazaki, Megumi Nakano, Kaori Saitoh, Harumi Hoshino, Niolette I. McGill, Howard Cooke, Hiroshi Masumoto

Research output: Contribution to journalArticle

335 Scopus citations

Abstract

To construct a mammalian artificial chromosome (MAC), telomere repeats and selectable markers were introduced into a 100 kb yeast artificial chromosome (YAC) containing human centromeric DNA. This YAC, which has a regular repeat structure of alpha-satellite DNA and centromere protein B (CENP-B) boxes, efficiently formed MACs that segregated accurately and bound CENP-B, CENP-C, and CENP-E. The MACs appear to be about 1-5 Mb in size and contain YAC multimers. Structural analyses suggest that the MACs have not acquired host sequences and were formed by a de novo mechanism. The accurate segregation of the MACs suggests they have potential as vectors for introducing genes into mammals.

Original languageEnglish (US)
Pages (from-to)431-439
Number of pages9
JournalNature Biotechnology
Volume16
Issue number5
DOIs
StatePublished - May 1998

Keywords

  • Alpha-satellite DNA
  • CENP-B boxes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

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  • Cite this

    Ikeno, M., Grimes, B., Okazaki, T., Nakano, M., Saitoh, K., Hoshino, H., McGill, N. I., Cooke, H., & Masumoto, H. (1998). Construction of YAC-based mammalian artificial chromosomes. Nature Biotechnology, 16(5), 431-439. https://doi.org/10.1038/nbt0598-431