CONTENTS OF SEVERAL AMINO ACIDS IN THE CEREBELLUM, BRAIN STEM AND CEREBRUM OF THE ‘STAGGERER’, ‘WEAVER’ AND ‘NERVOUS’ NEUROLOGICALLY MUTANT MICE

W. J. McBride, M. H. Aprison, K. Kusano

Research output: Contribution to journalArticle

92 Scopus citations

Abstract

The content of glutamate, GABA, aspartate, glycine and alanine was determined in the cerebellum, brain stem and cerebrum of three different mutant mice which have been named 'staggerer', 'weaver' and 'nervous' on the basis of neurological symptoms. In the 'staggerer' and 'weaver' mutants there is an almost complete absence of granule cells in the cerebellar cortex while in the 'nervous' mutant there is a loss of Purkinje cells (and to a lesser extent a loss of granule cells) in the cerebellar cortex. In the cerebellum of the 'weaver' mutant, the content of glutamate was significantly lower (P<0.025) than control values (8.77±0.76 vs 12.0±1.3 μmol/g tissue wet wt) and the contents of GABA and glycine were significantly greater than normal levels. In the cerebellum of the 'staggerer' mutant, the content of glutamate was significantly lower (6.62±0.70 μmol/g) and the contents of glycine and alanine significantly higher than control values. In the cerebrum and brain stem regions of the staggerer mutant, weaver mutant and the normals the contents of the five amino acids were the same. The contents of glycine and alanine in the cerebellum, GABA and glycine in the brain stem and GABA and alanine in the cerebrum of the nervous mutants were higher than control values. The data are discussed in terms of a possible role for glutamate functioning as an excitatory transmitter when released from the cerebellar granule cells.

Original languageEnglish (US)
Pages (from-to)867-870
Number of pages4
JournalJournal of Neurochemistry
Volume26
Issue number5
DOIs
StatePublished - May 1976

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'CONTENTS OF SEVERAL AMINO ACIDS IN THE CEREBELLUM, BRAIN STEM AND CEREBRUM OF THE ‘STAGGERER’, ‘WEAVER’ AND ‘NERVOUS’ NEUROLOGICALLY MUTANT MICE'. Together they form a unique fingerprint.

  • Cite this