Contribution of human CYP3A subfamily members to the 6-hydroxylation of chlorzoxazone

J. C. Gorski, D. R. Jones, S. A. Wrighton, S. D. Hall

Research output: Contribution to journalArticle

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Abstract

1. The capability of human CYPs other than 2E1 to catalyse the formation of 6-hydroxychlorzoxazone (6OHCHZ) was examined in vitro using human liver microsomes. 2. 4-Methylpyrazole, diethyldithiocarbamate (DDC), and rabbit anti-human CYP2E1 antibodies reduced chlorzoxazone 6-hydroxylase activity by 60, 60 and 50% respectively. The rate of formation of 6OHCHZ by DDC-treated microsomes was reduced further by the 3A inhibitors midazolam, troleandomycin and gestodene and increased by α-naphthoflavone, a 3A4 stimulator. 3. Following preincubation with DDC there were significant correlations (p < 0.05) between the residual CHZ 6-hydroxylase activity and immunoquantified CYP3A levels, and corresponding activities (e.g. midazolam 1'-hydroxylation). Rabbit anti-human CYP3A antibodies alone and in combination with DDC reduced the formation of 6OHCHZ by 47 and 62% respectively. 4. cDNA expressed CYP3A4, 2E1 and 2D6 exhibited comparable CHZ 6-hydroxylase activity. CHZ modulated 3A4 activity as reflected by midazolam 1'-hydroxylase and 4-hydroxylase activities. 5. CYP3A may make a significant contribution to CHZ 6-hydroxylation and therefore caution should be exercized when chlorzoxazone is employed as a specific 2E1 probe in vitro and in vivo.

Original languageEnglish
Pages (from-to)243-256
Number of pages14
JournalXenobiotica
Volume27
Issue number3
DOIs
StatePublished - 1997

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Chlorzoxazone
Cytochrome P-450 CYP3A
Hydroxylation
Ditiocarb
Mixed Function Oxygenases
Midazolam
Cytochrome P-450 CYP2E1
Troleandomycin
Rabbits
Antibodies
Liver Microsomes
Microsomes
Liver
Complementary DNA

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Pharmacology
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Contribution of human CYP3A subfamily members to the 6-hydroxylation of chlorzoxazone. / Gorski, J. C.; Jones, D. R.; Wrighton, S. A.; Hall, S. D.

In: Xenobiotica, Vol. 27, No. 3, 1997, p. 243-256.

Research output: Contribution to journalArticle

Gorski, J. C. ; Jones, D. R. ; Wrighton, S. A. ; Hall, S. D. / Contribution of human CYP3A subfamily members to the 6-hydroxylation of chlorzoxazone. In: Xenobiotica. 1997 ; Vol. 27, No. 3. pp. 243-256.
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