Contribution of hydrogen sulfide to the control of coronary blood flow

Eli D. Casalini, Adam G. Goodwill, Meredith K. Owen, Steven P. Moberly, Zachary C. Berwick, Johnathan D. Tune

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8 Scopus citations

Abstract

Objective: This study examined the mechanisms by which H2S modulates coronary microvascular resistance and myocardial perfusion at rest and in response to cardiac ischemia. Methods: Experiments were conducted in isolated coronary arteries and in open-chest anesthetized dogs. Results: We found that the H2S substrate l-cysteine (1-10 mM) did not alter coronary tone of isolated arteries in vitro or coronary blood flow in vivo. In contrast, intracoronary (ic) H2S (0.1-3 mM) increased coronary flow from 0.49 ± 0.08 to 2.65 ± 0.13 mL/min/g (p < 0.001). This increase in flow was unaffected by inhibition of Kv channels with 4-aminopyridine (p = 0.127) but was attenuated (0.23 ± 0.02-1.13 ± 0.13 mL/min/g) by the KATP channel antagonist glibenclamide (p < 0.001). Inhibition of NO synthesis (l-NAME) did not attenuate coronary responses to H2S. Immunohistochemistry revealed expression of CSE, an endogenous H2S enzyme, in myocardium. Inhibition of CSE with β-cyano-l-alanine (10 μM) had no effect on baseline coronary flow or responses to a 15-second coronary occlusion (p = 0.82). Conclusions: These findings demonstrate that exogenous H2S induces potent, endothelial-independent dilation of the coronary microcirculation predominantly through the activation of KATP channels, however, our data do not support a functional role for endogenous H2S in the regulation of coronary microvascular resistance.

Original languageEnglish (US)
Pages (from-to)104-111
Number of pages8
JournalMicrocirculation
Volume21
Issue number2
DOIs
StatePublished - Feb 1 2014

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Keywords

  • Coronary circulation
  • K channels
  • Reactive hyperemia

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)
  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

Contribution of hydrogen sulfide to the control of coronary blood flow. / Casalini, Eli D.; Goodwill, Adam G.; Owen, Meredith K.; Moberly, Steven P.; Berwick, Zachary C.; Tune, Johnathan D.

In: Microcirculation, Vol. 21, No. 2, 01.02.2014, p. 104-111.

Research output: Contribution to journalArticle