Control of granulopoiesis in man. III. Inhibition of colony formation by dense leukocytes

F. L. Baker, Hal Broxmeyer, P. R. Galbraith

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Cellular feeder layers, prepared from normal blood leukocytes, usually stimulate human marrow to form colonies. A significant increase in the stimulating activity of unseparated leukocyte feeder layers is brought about following the removal of dense leukocytes in a manner which avoids enrichment of any remaining cell type. Restoration of dense leukocytes to a dense leukocyte depleted leukocyte feeder layer results in the reduction of stimulating activity to that of an unseparated leukocyte feeder; however, addition of dense leukocytes to unseparated leukocyte feeder layers has no effect on the stimulatory activity, over the range of concentrations used in this study.

Original languageEnglish (US)
Pages (from-to)337-342
Number of pages6
JournalJournal of Cellular Physiology
Volume86
Issue number2 II
StatePublished - 1975
Externally publishedYes

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Feeder Cells
Leukocytes
Restoration
Blood
Bone Marrow

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Control of granulopoiesis in man. III. Inhibition of colony formation by dense leukocytes. / Baker, F. L.; Broxmeyer, Hal; Galbraith, P. R.

In: Journal of Cellular Physiology, Vol. 86, No. 2 II, 1975, p. 337-342.

Research output: Contribution to journalArticle

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