Control of mammalian glycogen synthase by PAS kinase

Wayne A. Wilson, Alexander V. Skurat, Brandon Probst, Anna De Paoli-Roach, Peter J. Roach, Jared Rutter

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

The regulation of glycogen metabolism is critical for the maintenance of glucose and energy homeostasis in mammals. Glycogen synthase, the enzyme responsible for glycogen production, is regulated by multisite phosphorylation in yeast and mammals. We have previously identified PAS kinase as a physiological regulator of glycogen synthase in Saccharomyces cerevisiae. We provide evidence here that PAS kinase is an important regulator of mammalian glycogen synthase. Glycogen synthase is efficiently phosphorylated by PAS kinase in vitro at Ser-540, a known regulatory phosphosite. Efficient phosphorylation requires a region of PAS kinase outside the catalytic domain. This region appears to mediate a direct interaction between glycogen synthase and PAS kinase, thereby targeting kinase activity to this substrate specifically. This interaction is regulated by the PAS kinase PAS domain, raising the possibility that this interaction (and phosphorylation event) is modulated by the cellular metabolic state. This mode of regulation provides a mechanism for metabolic status to impinge directly on the cellular decision of whether to store or use available energy.

Original languageEnglish (US)
Pages (from-to)16596-16601
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume102
Issue number46
DOIs
StatePublished - Nov 15 2005

Keywords

  • Phosphorylation

ASJC Scopus subject areas

  • Genetics
  • General

Fingerprint Dive into the research topics of 'Control of mammalian glycogen synthase by PAS kinase'. Together they form a unique fingerprint.

  • Cite this