Control of microtubule dynamics by the antagonistic activities of XMAP215 and XKCM1 in Xenopus egg extracts

Régis Tournebize, Andrei Popov, Kazuhisa Kinoshita, Anthony J. Ashford, Sonja Rybina, Andrei Pozniakovsky, Thomas U. Mayer, Claire Walczak, Eric Karsenti, Anthony A. Hyman

Research output: Contribution to journalArticle

248 Citations (Scopus)

Abstract

Microtubules are dynamic polymers that move stochastically between periods of growth and shrinkage, a property known as dynamic instability. Here, to investigate the mechanisms regulating microtubule dynamics in Xenopus egg extracts, we have cloned the complementary DNA encoding the microtubule-associated protein XMAP215 and investigated the function of the XMAP215 protein. Immunodepletion of XMAP215 indicated that it is a major microtubule-stabilizing factor in Xenopus egg extracts. During interphase, XMAP215 stabilizes microtubules primarily by opposing the activity of the destabilizing factor XKCM1, a member of the kinesin superfamily. These results indicate that microtubule dynamics in Xenopus egg extracts are regulated by a balance between a stabilizing factor, XMAP215, and a destabilizing factor, XKCM1.

Original languageEnglish
Pages (from-to)13-19
Number of pages7
JournalNature Cell Biology
Volume2
Issue number1
DOIs
StatePublished - 2000

Fingerprint

Xenopus
Microtubules
Ovum
Kinesin
Microtubule-Associated Proteins
Interphase
Polymers
Complementary DNA
Growth
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Tournebize, R., Popov, A., Kinoshita, K., Ashford, A. J., Rybina, S., Pozniakovsky, A., ... Hyman, A. A. (2000). Control of microtubule dynamics by the antagonistic activities of XMAP215 and XKCM1 in Xenopus egg extracts. Nature Cell Biology, 2(1), 13-19. https://doi.org/10.1038/71330

Control of microtubule dynamics by the antagonistic activities of XMAP215 and XKCM1 in Xenopus egg extracts. / Tournebize, Régis; Popov, Andrei; Kinoshita, Kazuhisa; Ashford, Anthony J.; Rybina, Sonja; Pozniakovsky, Andrei; Mayer, Thomas U.; Walczak, Claire; Karsenti, Eric; Hyman, Anthony A.

In: Nature Cell Biology, Vol. 2, No. 1, 2000, p. 13-19.

Research output: Contribution to journalArticle

Tournebize, R, Popov, A, Kinoshita, K, Ashford, AJ, Rybina, S, Pozniakovsky, A, Mayer, TU, Walczak, C, Karsenti, E & Hyman, AA 2000, 'Control of microtubule dynamics by the antagonistic activities of XMAP215 and XKCM1 in Xenopus egg extracts', Nature Cell Biology, vol. 2, no. 1, pp. 13-19. https://doi.org/10.1038/71330
Tournebize, Régis ; Popov, Andrei ; Kinoshita, Kazuhisa ; Ashford, Anthony J. ; Rybina, Sonja ; Pozniakovsky, Andrei ; Mayer, Thomas U. ; Walczak, Claire ; Karsenti, Eric ; Hyman, Anthony A. / Control of microtubule dynamics by the antagonistic activities of XMAP215 and XKCM1 in Xenopus egg extracts. In: Nature Cell Biology. 2000 ; Vol. 2, No. 1. pp. 13-19.
@article{8e83238d90a84c12aa23cea395a5fac5,
title = "Control of microtubule dynamics by the antagonistic activities of XMAP215 and XKCM1 in Xenopus egg extracts",
abstract = "Microtubules are dynamic polymers that move stochastically between periods of growth and shrinkage, a property known as dynamic instability. Here, to investigate the mechanisms regulating microtubule dynamics in Xenopus egg extracts, we have cloned the complementary DNA encoding the microtubule-associated protein XMAP215 and investigated the function of the XMAP215 protein. Immunodepletion of XMAP215 indicated that it is a major microtubule-stabilizing factor in Xenopus egg extracts. During interphase, XMAP215 stabilizes microtubules primarily by opposing the activity of the destabilizing factor XKCM1, a member of the kinesin superfamily. These results indicate that microtubule dynamics in Xenopus egg extracts are regulated by a balance between a stabilizing factor, XMAP215, and a destabilizing factor, XKCM1.",
author = "R{\'e}gis Tournebize and Andrei Popov and Kazuhisa Kinoshita and Ashford, {Anthony J.} and Sonja Rybina and Andrei Pozniakovsky and Mayer, {Thomas U.} and Claire Walczak and Eric Karsenti and Hyman, {Anthony A.}",
year = "2000",
doi = "10.1038/71330",
language = "English",
volume = "2",
pages = "13--19",
journal = "Nature Cell Biology",
issn = "1465-7392",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Control of microtubule dynamics by the antagonistic activities of XMAP215 and XKCM1 in Xenopus egg extracts

AU - Tournebize, Régis

AU - Popov, Andrei

AU - Kinoshita, Kazuhisa

AU - Ashford, Anthony J.

AU - Rybina, Sonja

AU - Pozniakovsky, Andrei

AU - Mayer, Thomas U.

AU - Walczak, Claire

AU - Karsenti, Eric

AU - Hyman, Anthony A.

PY - 2000

Y1 - 2000

N2 - Microtubules are dynamic polymers that move stochastically between periods of growth and shrinkage, a property known as dynamic instability. Here, to investigate the mechanisms regulating microtubule dynamics in Xenopus egg extracts, we have cloned the complementary DNA encoding the microtubule-associated protein XMAP215 and investigated the function of the XMAP215 protein. Immunodepletion of XMAP215 indicated that it is a major microtubule-stabilizing factor in Xenopus egg extracts. During interphase, XMAP215 stabilizes microtubules primarily by opposing the activity of the destabilizing factor XKCM1, a member of the kinesin superfamily. These results indicate that microtubule dynamics in Xenopus egg extracts are regulated by a balance between a stabilizing factor, XMAP215, and a destabilizing factor, XKCM1.

AB - Microtubules are dynamic polymers that move stochastically between periods of growth and shrinkage, a property known as dynamic instability. Here, to investigate the mechanisms regulating microtubule dynamics in Xenopus egg extracts, we have cloned the complementary DNA encoding the microtubule-associated protein XMAP215 and investigated the function of the XMAP215 protein. Immunodepletion of XMAP215 indicated that it is a major microtubule-stabilizing factor in Xenopus egg extracts. During interphase, XMAP215 stabilizes microtubules primarily by opposing the activity of the destabilizing factor XKCM1, a member of the kinesin superfamily. These results indicate that microtubule dynamics in Xenopus egg extracts are regulated by a balance between a stabilizing factor, XMAP215, and a destabilizing factor, XKCM1.

UR - http://www.scopus.com/inward/record.url?scp=0033792092&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033792092&partnerID=8YFLogxK

U2 - 10.1038/71330

DO - 10.1038/71330

M3 - Article

C2 - 10620801

AN - SCOPUS:0033792092

VL - 2

SP - 13

EP - 19

JO - Nature Cell Biology

JF - Nature Cell Biology

SN - 1465-7392

IS - 1

ER -