Controlled cervical laceration injury in mice.

Yi Ping Zhang, Melissa J. Walker, Lisa B.E. Shields, Xiaofei Wang, Chandler L. Walker, Xiao Ming Xu, Christopher B. Shields

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Use of genetically modified mice enhances our understanding of molecular mechanisms underlying several neurological disorders such as a spinal cord injury (SCI). Freehand manual control used to produce a laceration model of SCI creates inconsistent injuries often associated with a crush or contusion component and, therefore, a novel technique was developed. Our model of cervical laceration SCI has resolved inherent difficulties with the freehand method by incorporating 1) cervical vertebral stabilization by vertebral facet fixation, 2) enhanced spinal cord exposure, and 3) creation of a reproducible laceration of the spinal cord using an oscillating blade with an accuracy of ± 0.01 mm in depth without associated contusion. Compared to the standard methods of creating a SCI laceration such as freehand use of a scalpel or scissors, our method has produced a consistent lesion. This method is useful for studies on axonal regeneration of corticospinal, rubrospinal, and dorsal ascending tracts.

Original languageEnglish (US)
Pages (from-to)e50030
JournalJournal of visualized experiments : JoVE
Issue number75
StatePublished - 2013

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ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Zhang, Y. P., Walker, M. J., Shields, L. B. E., Wang, X., Walker, C. L., Xu, X. M., & Shields, C. B. (2013). Controlled cervical laceration injury in mice. Journal of visualized experiments : JoVE, (75), e50030.