Controlled cervical laceration injury in mice.

Yi Ping Zhang, Melissa J. Walker, Lisa B E Shields, Xiaofei Wang, Chandler Walker, Xiao-Ming Xu, Christopher B. Shields

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Use of genetically modified mice enhances our understanding of molecular mechanisms underlying several neurological disorders such as a spinal cord injury (SCI). Freehand manual control used to produce a laceration model of SCI creates inconsistent injuries often associated with a crush or contusion component and, therefore, a novel technique was developed. Our model of cervical laceration SCI has resolved inherent difficulties with the freehand method by incorporating 1) cervical vertebral stabilization by vertebral facet fixation, 2) enhanced spinal cord exposure, and 3) creation of a reproducible laceration of the spinal cord using an oscillating blade with an accuracy of ± 0.01 mm in depth without associated contusion. Compared to the standard methods of creating a SCI laceration such as freehand use of a scalpel or scissors, our method has produced a consistent lesion. This method is useful for studies on axonal regeneration of corticospinal, rubrospinal, and dorsal ascending tracts.

Original languageEnglish (US)
JournalJournal of visualized experiments : JoVE
Issue number75
StatePublished - 2013
Externally publishedYes

Fingerprint

Lacerations
Spinal Cord Injuries
Wounds and Injuries
Contusions
Manual control
Nervous System Diseases
Regeneration
Spinal Cord
Stabilization

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Zhang, Y. P., Walker, M. J., Shields, L. B. E., Wang, X., Walker, C., Xu, X-M., & Shields, C. B. (2013). Controlled cervical laceration injury in mice. Journal of visualized experiments : JoVE, (75).

Controlled cervical laceration injury in mice. / Zhang, Yi Ping; Walker, Melissa J.; Shields, Lisa B E; Wang, Xiaofei; Walker, Chandler; Xu, Xiao-Ming; Shields, Christopher B.

In: Journal of visualized experiments : JoVE, No. 75, 2013.

Research output: Contribution to journalArticle

Zhang, YP, Walker, MJ, Shields, LBE, Wang, X, Walker, C, Xu, X-M & Shields, CB 2013, 'Controlled cervical laceration injury in mice.', Journal of visualized experiments : JoVE, no. 75.
Zhang, Yi Ping ; Walker, Melissa J. ; Shields, Lisa B E ; Wang, Xiaofei ; Walker, Chandler ; Xu, Xiao-Ming ; Shields, Christopher B. / Controlled cervical laceration injury in mice. In: Journal of visualized experiments : JoVE. 2013 ; No. 75.
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