Convenient vectors for cloning and sequencing EcoRI and HindIII fragments

Howard J. Edenberg, Larry G. Moss, William J. Rutter

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

The polylinker regions of plasmid pUC and bacteriophage M13mp vectors have been specifically modified to provide alternative positions for cloning and reexcising EcoRI and HindIII fragments; the EcoRI and HindIII sites have been moved internal to BamHI and BglII sites. The location of EcoRI and HindIII sites in these HinEco vectors allows either.selective linearization or excision of the cloned fragments at unique flanking sites.

Original languageEnglish (US)
Pages (from-to)297-298
Number of pages2
JournalGene
Volume58
Issue number2-3
DOIs
StatePublished - 1987

Keywords

  • bacteriophage M13
  • multiple cloning site
  • plasmid pUC
  • polylinker
  • Recombinant DNA

ASJC Scopus subject areas

  • Genetics

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