Coordinate IGF-I and IGFBP5 gene expression in perinatal rat brain after hypoxia-ischemia

W. H. Lee, G. M. Wang, L. B. Seaman, S. J. Vannucci

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Insulin-like growth factor I (IGF-I) is an anabolic pleiotrophic factor essential for postnatal rat brain development, especially during the first 21 days, the 'critical growth period.' Cerebral hypoxic-ischemic insults occurring during the perinatal period can result in neuronal necrosis and permanent brain damage. To understand the regulation of the action of IGF-I in response to such a metabolic insult, we investigated the gene expression of IGF-I, type I IGF receptor, IGF binding protein (IGFBP) 2, and IGFBP5 during the first 72 h after hypoxia-ischemia in the immature rat. At 1 h of recovery, messenger RNA (mRNA) levels of all IGF system components were decreased throughout the hemisphere ipsilateral to the carotid artery ligation. This decrease is more pronounced at 24 h of recovery, especially in areas vulnerable to hypoxic-ischemic injury, such as the thalamus and hippocampus. At 72 h of recovery, although IGFBP2 and type 1 IGF receptor mRNA levels remain suppressed, gene expression of both IGF-I and IGFBP5 was activated in reactive astrocytes. Therefore, during the critical growth period in rats, the transcriptional levels of all IGF system components are extremely sensitive to metabolic perturbations associated with cerebral hypoxia-ischemia. The immediate decrease in IGF-I gene expression may be partially responsible for the impending neuronal death and selective vulnerability of myelinogenesis during the perinatal period.

Original languageEnglish
Pages (from-to)227-236
Number of pages10
JournalJournal of Cerebral Blood Flow and Metabolism
Volume16
Issue number2
StatePublished - 1996

Fingerprint

Brain Hypoxia-Ischemia
Insulin-Like Growth Factor I
Gene Expression
IGF Type 1 Receptor
Insulin-Like Growth Factor Binding Protein 2
Messenger RNA
Brain
Growth
Thalamus
Carotid Arteries
Astrocytes
Ligation
Hippocampus
Necrosis
Ischemia
Wounds and Injuries

Keywords

  • Brain injury
  • Gene expression
  • Hypoxia-ischemia
  • IGFBPs
  • Insulin-like growth factor I

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)
  • Endocrinology, Diabetes and Metabolism

Cite this

Coordinate IGF-I and IGFBP5 gene expression in perinatal rat brain after hypoxia-ischemia. / Lee, W. H.; Wang, G. M.; Seaman, L. B.; Vannucci, S. J.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 16, No. 2, 1996, p. 227-236.

Research output: Contribution to journalArticle

Lee, W. H. ; Wang, G. M. ; Seaman, L. B. ; Vannucci, S. J. / Coordinate IGF-I and IGFBP5 gene expression in perinatal rat brain after hypoxia-ischemia. In: Journal of Cerebral Blood Flow and Metabolism. 1996 ; Vol. 16, No. 2. pp. 227-236.
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