COPI transport complexes bind to specific RNAs in neuronal cells

Adrian G. Todd, Hai Lin, Allison D. Ebert, Yunlong Liu, Elliot Androphy

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Our fundamental understanding of how several thousand diverse RNAs are recognized in the soma, sorted, packaged, transported and localized within the cell is fragmentary. The COPa and COPb proteins of the coatomer protein I (COPI) vesicle complex were reported to interact with specific RNAs and represent a candidate RNA sorting and transport system. To determine the RNA-binding profile of Golgi-derived COPI in neuronal cells, we performed formaldehyde-linked RNA immunoprecipitation, followed by high-throughput sequencing, a process we term FLRIP-Seq (FLRIP, formaldehyde-cross-linked immunoprecipitation). We demonstrate that COPa co-immunoprecipitates a specific set of RNAs that are enriched in G-quadruplex motifs and fragile X mental retardation protein-associated RNAs and that encode factors that predominantly localize to the plasma membrane and cytoskeleton and function within signaling pathways. These data support the novel function of COPI in inter-compartmental trafficking of RNA.

Original languageEnglish
Article numberdds480
Pages (from-to)729-736
Number of pages8
JournalHuman Molecular Genetics
Volume22
Issue number4
DOIs
StatePublished - Feb 2013

Fingerprint

Coatomer Protein
Protein Transport
RNA
RNA Transport
Immunoprecipitation
Formaldehyde
Fragile X Mental Retardation Protein
G-Quadruplexes
Carisoprodol
Cytoskeleton
Cell Membrane

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)
  • Molecular Biology

Cite this

COPI transport complexes bind to specific RNAs in neuronal cells. / Todd, Adrian G.; Lin, Hai; Ebert, Allison D.; Liu, Yunlong; Androphy, Elliot.

In: Human Molecular Genetics, Vol. 22, No. 4, dds480, 02.2013, p. 729-736.

Research output: Contribution to journalArticle

Todd, Adrian G. ; Lin, Hai ; Ebert, Allison D. ; Liu, Yunlong ; Androphy, Elliot. / COPI transport complexes bind to specific RNAs in neuronal cells. In: Human Molecular Genetics. 2013 ; Vol. 22, No. 4. pp. 729-736.
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