Copy number determination of genetically-modified hematopoietic stem cells.

Todd Schuesler, Lilith Reeves, Christof von Kalle, Elke Grassman

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Human gene transfer with gammaretroviral, murine leukemia virus (MLV) based vectors has been shown to effectively insert and express transgene sequences at a level of therapeutic benefit. However, there are numerous reports of disruption of the normal cellular processes caused by the viral insertion, even of replication deficient gammaretroviral vectors. Current gammaretroviral and lentiviral vectors do not control the site of insertion into the genome, hence, the possibility of disruption of the target cell genome. Risk related to viral insertions is linked to the number of insertions of the transgene into the cellular DNA, as has been demonstrated for replication competent and replication deficient retroviruses in experiments. At high number of insertions per cell, cell transformation due to vector induced activation of proto-oncogenes is more likely to occur, in particular since more than one transforming event is needed for oncogenesis. Thus, determination of the vector copy number in bulk transduced populations, individual colony forming units, and tissue from the recipient of the transduced cells is an increasingly important safety assay and has become a standard, though not straightforward assay, since the inception of quantitative PCR.

Original languageEnglish (US)
Pages (from-to)281-298
Number of pages18
JournalMethods in molecular biology (Clifton, N.J.)
Volume506
StatePublished - 2009
Externally publishedYes

Fingerprint

Hematopoietic Stem Cells
Transgenes
Genome
Murine Leukemia Viruses
Proto-Oncogenes
Retroviridae
Carcinogenesis
Stem Cells
Safety
Polymerase Chain Reaction
DNA
Population
Genes
Therapeutics

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Schuesler, T., Reeves, L., Kalle, C. V., & Grassman, E. (2009). Copy number determination of genetically-modified hematopoietic stem cells. Methods in molecular biology (Clifton, N.J.), 506, 281-298.

Copy number determination of genetically-modified hematopoietic stem cells. / Schuesler, Todd; Reeves, Lilith; Kalle, Christof von; Grassman, Elke.

In: Methods in molecular biology (Clifton, N.J.), Vol. 506, 2009, p. 281-298.

Research output: Contribution to journalArticle

Schuesler, T, Reeves, L, Kalle, CV & Grassman, E 2009, 'Copy number determination of genetically-modified hematopoietic stem cells.', Methods in molecular biology (Clifton, N.J.), vol. 506, pp. 281-298.
Schuesler, Todd ; Reeves, Lilith ; Kalle, Christof von ; Grassman, Elke. / Copy number determination of genetically-modified hematopoietic stem cells. In: Methods in molecular biology (Clifton, N.J.). 2009 ; Vol. 506. pp. 281-298.
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