Corneal epithelial immune dendritic cell alterations in subtypes of dry eye disease: A pilot in vivo confocal microscopic study

Ahmad Kheirkhah, Raheleh Rahimi Darabad, Andrea Cruzat, Amir R. Hajrasouliha, Deborah Witkin, Nadia Wong, Reza Dana, Pedram Hamrah

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

PURPOSE. To evaluate density and morphology of corneal epithelial immune dendritic cells (DCs) in different subtypes of dry eye disease (DED) using in vivo confocal microscopy (IVCM). METHODS. This retrospective study included 59 eyes of 37 patients with DED and 40 eyes of 20 age-matched healthy controls. Based on clinical tests, eyes with DED were categorized into two subtypes: aqueous-deficient (n = 35) and evaporative (n = 24). For all subjects, images of laser scanning in vivo confocal microscopy (IVCM) of the central cornea were analyzed for DC density and DC morphology (DC size, number of dendrites, and DC field). These DC parameters were compared among all dry eye and control groups. RESULTS. Compared with the controls, patients with DED had significantly higher DC density, larger DC size, higher number of dendrites, and larger DC field (all P < 0.001). Comparison between aqueous-deficient and evaporative subtypes demonstrated that DC density was significantly higher in aqueous-deficient subtype (189.8 ± 36.9 vs. 58.9 ± 9.4 cells/mm 2 P = 0.001). However, there were no significant differences in morphologic parameters between DED subtypes. When aqueous-deficient DED with underlying systemic immune disease (Sj¨ogren’ s syndrome and graft versus host disease) were compared with nonimmune conditions, the immunologic subgroup showed significantly higher DC density, DC size, and number of dendrites (all P < 0.05). CONCLUSIONS. Corneal IVCM demonstrated differential changes in DC density and morphologic DC parameters between subtypes of DED. These changes, which reflect the degree of immune activation and inflammation in DED, can be used for clinical practice and endpoints in clinical trials.

Original languageEnglish (US)
Pages (from-to)7179-7185
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Volume56
Issue number12
DOIs
StatePublished - Nov 1 2015
Externally publishedYes

Fingerprint

Eye Diseases
Dendritic Cells
Cell Count
Dendrites
Cell Size
Confocal Microscopy
Sjogren's Syndrome
Immune System Diseases
Graft vs Host Disease
Cornea

Keywords

  • Dendritic cells
  • Dry eye disease
  • In vivo confocal microscopy
  • Inflammation

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Corneal epithelial immune dendritic cell alterations in subtypes of dry eye disease : A pilot in vivo confocal microscopic study. / Kheirkhah, Ahmad; Rahimi Darabad, Raheleh; Cruzat, Andrea; Hajrasouliha, Amir R.; Witkin, Deborah; Wong, Nadia; Dana, Reza; Hamrah, Pedram.

In: Investigative Ophthalmology and Visual Science, Vol. 56, No. 12, 01.11.2015, p. 7179-7185.

Research output: Contribution to journalArticle

Kheirkhah, Ahmad ; Rahimi Darabad, Raheleh ; Cruzat, Andrea ; Hajrasouliha, Amir R. ; Witkin, Deborah ; Wong, Nadia ; Dana, Reza ; Hamrah, Pedram. / Corneal epithelial immune dendritic cell alterations in subtypes of dry eye disease : A pilot in vivo confocal microscopic study. In: Investigative Ophthalmology and Visual Science. 2015 ; Vol. 56, No. 12. pp. 7179-7185.
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T1 - Corneal epithelial immune dendritic cell alterations in subtypes of dry eye disease

T2 - A pilot in vivo confocal microscopic study

AU - Kheirkhah, Ahmad

AU - Rahimi Darabad, Raheleh

AU - Cruzat, Andrea

AU - Hajrasouliha, Amir R.

AU - Witkin, Deborah

AU - Wong, Nadia

AU - Dana, Reza

AU - Hamrah, Pedram

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N2 - PURPOSE. To evaluate density and morphology of corneal epithelial immune dendritic cells (DCs) in different subtypes of dry eye disease (DED) using in vivo confocal microscopy (IVCM). METHODS. This retrospective study included 59 eyes of 37 patients with DED and 40 eyes of 20 age-matched healthy controls. Based on clinical tests, eyes with DED were categorized into two subtypes: aqueous-deficient (n = 35) and evaporative (n = 24). For all subjects, images of laser scanning in vivo confocal microscopy (IVCM) of the central cornea were analyzed for DC density and DC morphology (DC size, number of dendrites, and DC field). These DC parameters were compared among all dry eye and control groups. RESULTS. Compared with the controls, patients with DED had significantly higher DC density, larger DC size, higher number of dendrites, and larger DC field (all P < 0.001). Comparison between aqueous-deficient and evaporative subtypes demonstrated that DC density was significantly higher in aqueous-deficient subtype (189.8 ± 36.9 vs. 58.9 ± 9.4 cells/mm 2 P = 0.001). However, there were no significant differences in morphologic parameters between DED subtypes. When aqueous-deficient DED with underlying systemic immune disease (Sj¨ogren’ s syndrome and graft versus host disease) were compared with nonimmune conditions, the immunologic subgroup showed significantly higher DC density, DC size, and number of dendrites (all P < 0.05). CONCLUSIONS. Corneal IVCM demonstrated differential changes in DC density and morphologic DC parameters between subtypes of DED. These changes, which reflect the degree of immune activation and inflammation in DED, can be used for clinical practice and endpoints in clinical trials.

AB - PURPOSE. To evaluate density and morphology of corneal epithelial immune dendritic cells (DCs) in different subtypes of dry eye disease (DED) using in vivo confocal microscopy (IVCM). METHODS. This retrospective study included 59 eyes of 37 patients with DED and 40 eyes of 20 age-matched healthy controls. Based on clinical tests, eyes with DED were categorized into two subtypes: aqueous-deficient (n = 35) and evaporative (n = 24). For all subjects, images of laser scanning in vivo confocal microscopy (IVCM) of the central cornea were analyzed for DC density and DC morphology (DC size, number of dendrites, and DC field). These DC parameters were compared among all dry eye and control groups. RESULTS. Compared with the controls, patients with DED had significantly higher DC density, larger DC size, higher number of dendrites, and larger DC field (all P < 0.001). Comparison between aqueous-deficient and evaporative subtypes demonstrated that DC density was significantly higher in aqueous-deficient subtype (189.8 ± 36.9 vs. 58.9 ± 9.4 cells/mm 2 P = 0.001). However, there were no significant differences in morphologic parameters between DED subtypes. When aqueous-deficient DED with underlying systemic immune disease (Sj¨ogren’ s syndrome and graft versus host disease) were compared with nonimmune conditions, the immunologic subgroup showed significantly higher DC density, DC size, and number of dendrites (all P < 0.05). CONCLUSIONS. Corneal IVCM demonstrated differential changes in DC density and morphologic DC parameters between subtypes of DED. These changes, which reflect the degree of immune activation and inflammation in DED, can be used for clinical practice and endpoints in clinical trials.

KW - Dendritic cells

KW - Dry eye disease

KW - In vivo confocal microscopy

KW - Inflammation

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