Correlating colorectal cancer risk with field carcinogenesis progression using partial wave spectroscopic microscopy

Scott Gladstein, Dhwanil Damania, Luay M. Almassalha, Lauren T. Smith, Varun Gupta, Hariharan Subramanian, Douglas Rex, Hemant K. Roy, Vadim Backman

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Prior to the development of a localized cancerous tumor, diffuse molecular, and structural alterations occur throughout an organ due to genetic, environmental, and lifestyle factors. This process is known as field carcinogenesis. In this study, we used partial wave spectroscopic (PWS) microscopy to explore the progression of field carcinogenesis by measuring samples collected from 190 patients with a range of colonic history (no history, low-risk history, and high-risk history) and current colon health (healthy, nondiminutive adenomas (NDA; ≥5 mm and <10 mm), and advanced adenoma [AA; ≥10 mm, HGD, or >25% villous features]). The low-risk history groups include patients with a history of NDA. The high-risk history groups include patients with either a history of AA or colorectal cancer (CRC). PWS is a nanoscale-sensitive imaging technique which measures the organization of intracellular structure. Previous studies have shown that PWS is sensitive to changes in the higher-order (20-200 nm) chromatin topology that occur due to field carcinogenesis within histologically normal cells. The results of this study show that these nanoscale structural alterations are correlated with a patient's colonic history, which suggests that PWS can detect altered field carcinogenic signatures even in patients with negative colonoscopies. Furthermore, we developed a model to calculate the 5-year risk of developing CRC for each patient group. We found that our data fit this model remarkably well (R2 = 0.946). This correlation suggests that PWS could potentially be used to monitor CRC progression less invasively and in patients without adenomas, which opens PWS to many potential cancer care applications.

Original languageEnglish (US)
JournalCancer Medicine
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Microscopy
Colorectal Neoplasms
Carcinogenesis
History
Adenoma
Colonoscopy
Chromatin
Life Style
Neoplasms
Colon
Health

Keywords

  • Cancer risk
  • Chromatin
  • Colorectal cancer
  • Field carcinogenesis
  • Partial wave spectroscopic microscopy

ASJC Scopus subject areas

  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Cancer Research

Cite this

Gladstein, S., Damania, D., Almassalha, L. M., Smith, L. T., Gupta, V., Subramanian, H., ... Backman, V. (Accepted/In press). Correlating colorectal cancer risk with field carcinogenesis progression using partial wave spectroscopic microscopy. Cancer Medicine. https://doi.org/10.1002/cam4.1357

Correlating colorectal cancer risk with field carcinogenesis progression using partial wave spectroscopic microscopy. / Gladstein, Scott; Damania, Dhwanil; Almassalha, Luay M.; Smith, Lauren T.; Gupta, Varun; Subramanian, Hariharan; Rex, Douglas; Roy, Hemant K.; Backman, Vadim.

In: Cancer Medicine, 01.01.2018.

Research output: Contribution to journalArticle

Gladstein, S, Damania, D, Almassalha, LM, Smith, LT, Gupta, V, Subramanian, H, Rex, D, Roy, HK & Backman, V 2018, 'Correlating colorectal cancer risk with field carcinogenesis progression using partial wave spectroscopic microscopy', Cancer Medicine. https://doi.org/10.1002/cam4.1357
Gladstein, Scott ; Damania, Dhwanil ; Almassalha, Luay M. ; Smith, Lauren T. ; Gupta, Varun ; Subramanian, Hariharan ; Rex, Douglas ; Roy, Hemant K. ; Backman, Vadim. / Correlating colorectal cancer risk with field carcinogenesis progression using partial wave spectroscopic microscopy. In: Cancer Medicine. 2018.
@article{aa881c5e11634b8891194a49a8c59434,
title = "Correlating colorectal cancer risk with field carcinogenesis progression using partial wave spectroscopic microscopy",
abstract = "Prior to the development of a localized cancerous tumor, diffuse molecular, and structural alterations occur throughout an organ due to genetic, environmental, and lifestyle factors. This process is known as field carcinogenesis. In this study, we used partial wave spectroscopic (PWS) microscopy to explore the progression of field carcinogenesis by measuring samples collected from 190 patients with a range of colonic history (no history, low-risk history, and high-risk history) and current colon health (healthy, nondiminutive adenomas (NDA; ≥5 mm and <10 mm), and advanced adenoma [AA; ≥10 mm, HGD, or >25{\%} villous features]). The low-risk history groups include patients with a history of NDA. The high-risk history groups include patients with either a history of AA or colorectal cancer (CRC). PWS is a nanoscale-sensitive imaging technique which measures the organization of intracellular structure. Previous studies have shown that PWS is sensitive to changes in the higher-order (20-200 nm) chromatin topology that occur due to field carcinogenesis within histologically normal cells. The results of this study show that these nanoscale structural alterations are correlated with a patient's colonic history, which suggests that PWS can detect altered field carcinogenic signatures even in patients with negative colonoscopies. Furthermore, we developed a model to calculate the 5-year risk of developing CRC for each patient group. We found that our data fit this model remarkably well (R2 = 0.946). This correlation suggests that PWS could potentially be used to monitor CRC progression less invasively and in patients without adenomas, which opens PWS to many potential cancer care applications.",
keywords = "Cancer risk, Chromatin, Colorectal cancer, Field carcinogenesis, Partial wave spectroscopic microscopy",
author = "Scott Gladstein and Dhwanil Damania and Almassalha, {Luay M.} and Smith, {Lauren T.} and Varun Gupta and Hariharan Subramanian and Douglas Rex and Roy, {Hemant K.} and Vadim Backman",
year = "2018",
month = "1",
day = "1",
doi = "10.1002/cam4.1357",
language = "English (US)",
journal = "Cancer Medicine",
issn = "2045-7634",
publisher = "John Wiley and Sons Ltd",

}

TY - JOUR

T1 - Correlating colorectal cancer risk with field carcinogenesis progression using partial wave spectroscopic microscopy

AU - Gladstein, Scott

AU - Damania, Dhwanil

AU - Almassalha, Luay M.

AU - Smith, Lauren T.

AU - Gupta, Varun

AU - Subramanian, Hariharan

AU - Rex, Douglas

AU - Roy, Hemant K.

AU - Backman, Vadim

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Prior to the development of a localized cancerous tumor, diffuse molecular, and structural alterations occur throughout an organ due to genetic, environmental, and lifestyle factors. This process is known as field carcinogenesis. In this study, we used partial wave spectroscopic (PWS) microscopy to explore the progression of field carcinogenesis by measuring samples collected from 190 patients with a range of colonic history (no history, low-risk history, and high-risk history) and current colon health (healthy, nondiminutive adenomas (NDA; ≥5 mm and <10 mm), and advanced adenoma [AA; ≥10 mm, HGD, or >25% villous features]). The low-risk history groups include patients with a history of NDA. The high-risk history groups include patients with either a history of AA or colorectal cancer (CRC). PWS is a nanoscale-sensitive imaging technique which measures the organization of intracellular structure. Previous studies have shown that PWS is sensitive to changes in the higher-order (20-200 nm) chromatin topology that occur due to field carcinogenesis within histologically normal cells. The results of this study show that these nanoscale structural alterations are correlated with a patient's colonic history, which suggests that PWS can detect altered field carcinogenic signatures even in patients with negative colonoscopies. Furthermore, we developed a model to calculate the 5-year risk of developing CRC for each patient group. We found that our data fit this model remarkably well (R2 = 0.946). This correlation suggests that PWS could potentially be used to monitor CRC progression less invasively and in patients without adenomas, which opens PWS to many potential cancer care applications.

AB - Prior to the development of a localized cancerous tumor, diffuse molecular, and structural alterations occur throughout an organ due to genetic, environmental, and lifestyle factors. This process is known as field carcinogenesis. In this study, we used partial wave spectroscopic (PWS) microscopy to explore the progression of field carcinogenesis by measuring samples collected from 190 patients with a range of colonic history (no history, low-risk history, and high-risk history) and current colon health (healthy, nondiminutive adenomas (NDA; ≥5 mm and <10 mm), and advanced adenoma [AA; ≥10 mm, HGD, or >25% villous features]). The low-risk history groups include patients with a history of NDA. The high-risk history groups include patients with either a history of AA or colorectal cancer (CRC). PWS is a nanoscale-sensitive imaging technique which measures the organization of intracellular structure. Previous studies have shown that PWS is sensitive to changes in the higher-order (20-200 nm) chromatin topology that occur due to field carcinogenesis within histologically normal cells. The results of this study show that these nanoscale structural alterations are correlated with a patient's colonic history, which suggests that PWS can detect altered field carcinogenic signatures even in patients with negative colonoscopies. Furthermore, we developed a model to calculate the 5-year risk of developing CRC for each patient group. We found that our data fit this model remarkably well (R2 = 0.946). This correlation suggests that PWS could potentially be used to monitor CRC progression less invasively and in patients without adenomas, which opens PWS to many potential cancer care applications.

KW - Cancer risk

KW - Chromatin

KW - Colorectal cancer

KW - Field carcinogenesis

KW - Partial wave spectroscopic microscopy

UR - http://www.scopus.com/inward/record.url?scp=85044297841&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85044297841&partnerID=8YFLogxK

U2 - 10.1002/cam4.1357

DO - 10.1002/cam4.1357

M3 - Article

C2 - 29573208

AN - SCOPUS:85044297841

JO - Cancer Medicine

JF - Cancer Medicine

SN - 2045-7634

ER -