Correlation between CSF-1 responsiveness and expression of (CSF-1 receptor) c-fms in purified murine granulocyte-macrophage progenitor cells (CFU-GM)

D. C. Bicknell, D. E. Williams, H. E. Broxmeyer

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The gene product of the viral proto-oncogene v-fms, associated with the feline sarcoma virus (SM-FeSV), is a glycoprotein of 170 kd with associated tyrosine kinase activity. The murine c-fms proto-oncogene produces a similar glycoprotein of 165 kd and has been implicated as a similar, if not identical, molecule to the cell surface receptor of the hematopoietic growth factor CSF-1. Employing a v-fms probe in an in situ hybridization assay we examined the expression of c-fms transcripts in a population of purified granulocyte-macrophage progenitor cells (CFU-GM). Using this approach, which requires only 10-20 thousand cells, we demonstrate that 53% of the cells in the purified CFU-GM containing fraction (FR-28) express c-fms transcripts. In the presence of natural purified CSF-1, 51% ± 3% of FR-28 cells form colonies or clusters in a semi-solid culture assay system. 94% of the colonies and clusters stimulated by CSF-1 were morphologically macrophage in nature. In addition, 84% ± 4% of FR-28 cells formed colonies and clusters when stimulated with pokeweed mitogen spleen cell conditioned medium (PWMSCM) in a similar in vitro assay (35% granulocyte, 29% macrophage and 39% mixed granulocyte/macrophage colonies). These results indicate there is a correlation between the responsiveness of CFU-GM progenitor cells to CSF-1 and the expression of c-fms RNA.

Original languageEnglish (US)
Pages (from-to)240-243
Number of pages4
JournalExperimental Hematology
Volume16
Issue number3
StatePublished - Jan 1 1988

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Macrophage Colony-Stimulating Factor Receptors
Granulocyte-Macrophage Progenitor Cells
Macrophage Colony-Stimulating Factor
Macrophages
Granulocytes
Feline Sarcoma Viruses
Glycoproteins
fms Genes
Pokeweed Mitogens
Proto-Oncogenes
Cell Surface Receptors
Viral Proteins
Conditioned Culture Medium
Protein-Tyrosine Kinases
In Situ Hybridization
Intercellular Signaling Peptides and Proteins
Stem Cells
Spleen
RNA
Population

ASJC Scopus subject areas

  • Molecular Biology
  • Hematology
  • Genetics
  • Cell Biology
  • Cancer Research

Cite this

Correlation between CSF-1 responsiveness and expression of (CSF-1 receptor) c-fms in purified murine granulocyte-macrophage progenitor cells (CFU-GM). / Bicknell, D. C.; Williams, D. E.; Broxmeyer, H. E.

In: Experimental Hematology, Vol. 16, No. 3, 01.01.1988, p. 240-243.

Research output: Contribution to journalArticle

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abstract = "The gene product of the viral proto-oncogene v-fms, associated with the feline sarcoma virus (SM-FeSV), is a glycoprotein of 170 kd with associated tyrosine kinase activity. The murine c-fms proto-oncogene produces a similar glycoprotein of 165 kd and has been implicated as a similar, if not identical, molecule to the cell surface receptor of the hematopoietic growth factor CSF-1. Employing a v-fms probe in an in situ hybridization assay we examined the expression of c-fms transcripts in a population of purified granulocyte-macrophage progenitor cells (CFU-GM). Using this approach, which requires only 10-20 thousand cells, we demonstrate that 53{\%} of the cells in the purified CFU-GM containing fraction (FR-28) express c-fms transcripts. In the presence of natural purified CSF-1, 51{\%} ± 3{\%} of FR-28 cells form colonies or clusters in a semi-solid culture assay system. 94{\%} of the colonies and clusters stimulated by CSF-1 were morphologically macrophage in nature. In addition, 84{\%} ± 4{\%} of FR-28 cells formed colonies and clusters when stimulated with pokeweed mitogen spleen cell conditioned medium (PWMSCM) in a similar in vitro assay (35{\%} granulocyte, 29{\%} macrophage and 39{\%} mixed granulocyte/macrophage colonies). These results indicate there is a correlation between the responsiveness of CFU-GM progenitor cells to CSF-1 and the expression of c-fms RNA.",
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