Correlation of Qa-1 determinants defined by antisera and by cytotoxic T lymphocytes

Robert N. Jenkins, Carla Aldrich, Nicholas F. Landolfi, Robert R. Rich

Research output: Contribution to journalArticle

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Abstract

Cytotoxic T lymphocytes (CTL) activated in H-2 identical, Qa-1 disparate mixed leukocyte cultures recognize H-2-nonrestricted target antigens indistinguishable by strain or tissue distribution from serologically defined Qa-1 antigens. Cloned Qa-l-specific CTL define determinants encoded by four Qa-1 genotypes; we used anti-Qa-1 sera in antibody blocking experiments to determine if these determinants reside on molecules recognized by Qa-1-specific antibodies. Antisera containing Qa-1.1-specific and TL-specific antibodies blocked recognition of two CTL-defined determinants associated with Qa-1a. Although both Qa-1 and TL molecules are expressed on activated T cells from appropriate strains, our studies indicated that the CTL recognized Qa-1, not TL. In addition, anti-Qa-1.2 serum inhibited CTL recognition of Qa-1b- and Qa-1c-encoded determinants. Qa-1d target cells are unique in that they express determinants recognized by anti-Qa-1a CTL and by anti-Qa-1b CTL. Killing of Qa-1d targets by anti-Qa-1a CTL was not inhibited by anti-Qa-1.1 serum, but was partially inhibited by anti-Qa-1.2 serum. Cytotoxicity of Qa-1d cells by one anti-Qa-1b CTL clone was inhibited by both anti-Qa-1.2 and anti-Qa-1.1 sera, indicating close association of both serological determinants with the determinants recognized by the CTL. Thus, all of the CTL-defined Qa-1 determinants resided on molecules recognized by Qa-1-specific antibodies, but anti-Qa-1a CTL and Qa-1.1-specific antibodies did not have identical specificities.

Original languageEnglish (US)
Pages (from-to)215-225
Number of pages11
JournalImmunogenetics
Volume21
Issue number3
DOIs
StatePublished - Mar 1985
Externally publishedYes

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Cytotoxic T-Lymphocytes
Immune Sera
Serum
Antibodies
Blocking Antibodies
Tissue Distribution
Anti-Idiotypic Antibodies
Leukocytes
Clone Cells
Genotype
T-Lymphocytes
Antigens

ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this

Correlation of Qa-1 determinants defined by antisera and by cytotoxic T lymphocytes. / Jenkins, Robert N.; Aldrich, Carla; Landolfi, Nicholas F.; Rich, Robert R.

In: Immunogenetics, Vol. 21, No. 3, 03.1985, p. 215-225.

Research output: Contribution to journalArticle

Jenkins, Robert N. ; Aldrich, Carla ; Landolfi, Nicholas F. ; Rich, Robert R. / Correlation of Qa-1 determinants defined by antisera and by cytotoxic T lymphocytes. In: Immunogenetics. 1985 ; Vol. 21, No. 3. pp. 215-225.
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