Cortical bone mechanical properties are altered in an animal model of progressive chronic kidney disease

Christopher L. Newman, Sharon Moe, Xuening (Neal) Chen, Max A. Hammond, Joseph M. Wallace, Jeffry S. Nyman, Matthew Allen

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Chronic kidney disease (CKD), which leads tocortical bone loss and increasedporosity,increases therisk of fracture. Animal models have confirmed that these changes compromise whole bone mechanical properties. Estimates from whole bone testing suggest that material properties are negatively affected, though tissue-level assessmentshavenot been conducted. Therefore, the goal of the present study was to examine changes in cortical bone at different length scales using a rat model with theprogressive development of CKD. At 30 weeks of age (∼75% reduction in kidney function), skeletally mature male Cy/+ rats were compared to their normal littermates. Cortical bone material propertieswere assessed with reference point indentation (RPI), atomic force microscopy (AFM), Raman spectroscopy,and high performance liquid chromatography (HPLC). Bones from animals with CKD had higher (+18%) indentation distance increase and first cycle energy dissipation (+ 8%) as measured by RPI.AFM indentation revealed a broader distribution of elastic modulus values in CKD animals witha greater proportion of both higher and lower modulus values compared to normal controls. Yet, tissue composition, collagen morphology, and collagen cross-linking fail to account for these differences. Though the specific skeletal tissue alterations responsible for these mechanical differences remain unclear, these results indicate that cortical bone material properties are altered in these animals and may contribute to the increased fracture risk associated with CKD.

Original languageEnglish
Article numbere99262
JournalPLoS One
Volume9
Issue number6
DOIs
StatePublished - Jun 9 2014

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kidney diseases
Chronic Renal Insufficiency
mechanical properties
Bone
Animals
Animal Models
animal models
bones
Mechanical properties
Indentation
Bone and Bones
Atomic Force Microscopy
atomic force microscopy
Collagen
Tissue
Materials Testing
collagen
Rats
Atomic force microscopy
Materials properties

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Cortical bone mechanical properties are altered in an animal model of progressive chronic kidney disease. / Newman, Christopher L.; Moe, Sharon; Chen, Xuening (Neal); Hammond, Max A.; Wallace, Joseph M.; Nyman, Jeffry S.; Allen, Matthew.

In: PLoS One, Vol. 9, No. 6, e99262, 09.06.2014.

Research output: Contribution to journalArticle

Newman, Christopher L. ; Moe, Sharon ; Chen, Xuening (Neal) ; Hammond, Max A. ; Wallace, Joseph M. ; Nyman, Jeffry S. ; Allen, Matthew. / Cortical bone mechanical properties are altered in an animal model of progressive chronic kidney disease. In: PLoS One. 2014 ; Vol. 9, No. 6.
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