Corticosteroids block newly induced but not constitutive functions of macrophage cell lines: myeloid colony-stimulating activity production, latex phagocytosis, and antibody-dependent lysis of RBC and tumor targets

P. Ralph, M. Ito, H. E. Broxmeyer, I. Nakoinz

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Abstract

Hydrocortisone at 10-4 M added to macrophage cell lines had no effect on the following activities: J774.1 antibody-dependent phagocytosis and lysis of sheep RBC, RAW 264 antibody-dependent lysis of tumor targets, latex bead phagocytosis by PU5-1.8 cells, and constitutive production of myeloid colony-stimulating activity (CSA) by WEHI-3 cells. In contrast, 10-4 M hydrocortisone completely inhibited LPS or PPD activation of the following functions: induced production of myeloid CSA, stimulation of latex phagocytosis and stimulation of antibody-dependent lysis of tumor targets by PU5-1.8 cell line, and stimulation of antibody-dependent lysis of RBC targets by RAW 264 cell line. Lower concentrations of hydrocortisone or dexamethasone at 10-6 M inhibited the LPS induction of CSA from 55 to 90%. Growth inhibition of PU5-1.8 cells caused by 1 μg/ml LPS was also partially reversed by corticosteroids. Thus, steroids block these newly induced but not constitutive functions of these macrophage cells in culture.

Original languageEnglish (US)
Pages (from-to)300-303
Number of pages4
JournalJournal of Immunology
Volume121
Issue number1
StatePublished - Dec 1 1978

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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