Covalent Interaction of Dehydroretronecine, a Carcinogenic Metabolite of the Pyrrolizidine Alkaloid Monocrotaline, with Cysteine and Glutathione

K. A. Robertson, J. L. Seymour, M. T. Hsia, J. R. Allen

Research output: Contribution to journalArticle

52 Scopus citations

Abstract

The covalent interaction of dehydroretronecine, a carcinogenic metabolite of the pyrrolizidine alkaloid monocrotaline, with cysteine and glutathione, has been investigated. Dehydroretronecine was allowed to react with cysteine and glutathione in an in vitro system of phosphate buffer solutions. The reaction products were identified structurally by chromatographic, nuclear magnetic resonance, infrared, ultraviolet, and mass-spectral analysis. These data indicate that the reaction products are the sulfhydryl-linked 7-thiocysteine-dehydroretronecine and 7-thioglutathione-dehydro-retronecine. Active alkylation of sulfhydryl groups is a possible mechanism by which these alkaloids interact with cellular components.

Original languageEnglish (US)
Pages (from-to)3141-3144
Number of pages4
JournalCancer Research
Volume37
Issue number9
StatePublished - Sep 1977
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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