Creatine kinase isoenzymes are highly regulated during pregnancy in rat uterus and placenta

R. Payne, D. L. Friedman, J. W. Grant, M. B. Perryman, A. W. Strauss

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Creatine kinase (CK) isoenzymes play a central role in energy transfer. Expression of CK isoenzymes in rat uterus and placenta was analyzed to evaluate their contribution to energy metabolism during pregnancy and delivery. Tissue from the uterine horns and placentas of pregnant rats from day 14 of gestation to 17 days postpartum was analyzed for expression of 'brain' CK (BCK) and ubiquitous mitochondrial CK (uMtCK) mRNA, protein, and enzyme activity. uMtCK mRNA expression is high in prepartum uterus, but rapidly falls (>10-fold) after delivery to a nadir at 7 days postpartum. Prepartum BCK mRNA expression is coordinate with uMtCK but has a 15-fold greater expression than uMtCK. Both CK mRNAs rise by 17 days postpartum. Both BCK and uMtCK mRNA expressions are strongly induced in placenta at 20 days gestation with a rapid fall (>6-fold) immediately before delivery. Protein expression of BCK and uMtCK is also coordinate. However, analysis of mRNA and protein expression indicates that significant posttranscriptional regulation of both kinds of CK also occurs. CK activity in uterus and placenta reflects BCK expression. By immunohistochemistry, BCK and uMtCK protein expression is highly localized in the placenta and endometrium of prepartum uterus. This expression shifts entirely to the uterine smooth muscle by 17 days postpartum. uMtCK mRNA expression is rapidly induced by β-estradiol in vitro (>6-fold), demonstrating estrogen-responsive elements in the uMtCK nuclear gene. Thus a second isoenzyme of CK, uMtCK, is expressed in rat uterus and placenta and is highly regulated with BCK. These results suggest an important role for CK in the maintenance and termination of pregnancy.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume265
Issue number4 28-4
StatePublished - 1993
Externally publishedYes

Fingerprint

Creatine Kinase
Placenta
Isoenzymes
Uterus
Rats
Brain
Pregnancy
Messenger RNA
Postpartum Period
Mitochondrial Form Creatine Kinase
BB Form Creatine Kinase
Pregnancy Maintenance
Proteins
Myometrium
Mitochondrial Proteins
Energy Transfer
Endometrium
Enzyme activity
Energy Metabolism
Smooth Muscle

Keywords

  • endometrium
  • metabolism
  • smooth muscle

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology

Cite this

Creatine kinase isoenzymes are highly regulated during pregnancy in rat uterus and placenta. / Payne, R.; Friedman, D. L.; Grant, J. W.; Perryman, M. B.; Strauss, A. W.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 265, No. 4 28-4, 1993.

Research output: Contribution to journalArticle

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