Cross-calibration of 45calcium kinetics against dynamic histomorphometry in a rat model to determine bone turnover

Mohammad Shahnazari, David Burr, Wang Hee Lee, Berdine R. Martin, Connie M. Weaver

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Techniques for assessing bone dynamic are in high demand. Calcium (Ca) kinetic studies are currently being used in our clinical studies of bone turnover in adolescents and elderly. The technique has rarely been compared to the standard method of bone dynamic histomorphometry. We perturbed bone turnover through ovariectomy and sub-optimal dietary Ca in a female rat model to cross-calibrate Ca kinetics against dynamic histomorphometry. Kinetic studies involved oral and intravenous administration of 45Ca and monitoring the tracer in blood, urine, feces, and bone over a 3-day period as part of a metabolic Ca balance study. Histomorphometric indices of mineral apposition rate, mineralizing surface, and bone formation rate were obtained from proximal metaphysis and mid-diaphysis region of tibial bone. Bone mineralization and resorption rates at the whole skeletal level as evaluated by kinetic studies were significantly correlated with the volume-based bone formation rate (BFR/BV) evaluated by dynamic histomorphometry in metaphyseal trabecular bone (r=0.72 and r=0.61, respectively, p<0.001) and surface-based bone formation rate (BFR/BS) in tibial cortex (r=0.63, p<0.001 and r=0.59, p<0.01, respectively). Significant correlations were also demonstrated between bone resorption and mineralization rates at the whole skeletal level (r=0.91, p<0.001) using 45Ca kinetic data. Ca kinetic modeling showed an increase (p<0.001) in skeletal resorption and formation rates in response to ovariectomy (27.6 vs. 13.8mg/d for bone resorption and 42.7 vs. 28mg/d for bone formation in ovariectomized vs. their Sham-operated control animals, respectively). Ca kinetic data also showed that bone formation decreased by 30% and whole bone balance by 50%, when dietary Ca level was reduced from 0.4% to 0.2% (34.2 vs. 23.8mg/d and 10.4 vs. 5.1mg/d, respectively, p<0.001). Our data suggest that Ca kinetic studies can be used reliably to rapidly detect changes in bone turnover at the whole skeletal level in response to interventions.

Original languageEnglish
Pages (from-to)1238-1243
Number of pages6
JournalBone
Volume46
Issue number5
DOIs
StatePublished - May 2010

Fingerprint

Bone Remodeling
Calibration
Osteogenesis
Calcium
Bone and Bones
Bone Resorption
Dietary Calcium
Physiologic Calcification
Ovariectomy
Diaphyses
Feces
Intravenous Administration
Minerals
Oral Administration
Urine

Keywords

  • Bone remodeling
  • Bone turnover
  • Calcium kinetics
  • Dynamic histomorphometry

ASJC Scopus subject areas

  • Physiology
  • Endocrinology, Diabetes and Metabolism
  • Histology

Cite this

Cross-calibration of 45calcium kinetics against dynamic histomorphometry in a rat model to determine bone turnover. / Shahnazari, Mohammad; Burr, David; Lee, Wang Hee; Martin, Berdine R.; Weaver, Connie M.

In: Bone, Vol. 46, No. 5, 05.2010, p. 1238-1243.

Research output: Contribution to journalArticle

Shahnazari, Mohammad ; Burr, David ; Lee, Wang Hee ; Martin, Berdine R. ; Weaver, Connie M. / Cross-calibration of 45calcium kinetics against dynamic histomorphometry in a rat model to determine bone turnover. In: Bone. 2010 ; Vol. 46, No. 5. pp. 1238-1243.
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