Cross-reaction of albumin with polyclonal lh antibody on western blots

M. R. Kelley, M. A. Emanuele, J. Tentler, N. V. Emanuele

Research output: Contribution to journalArticle

Abstract

When pituitary tissue was subjected to Western blot analysis utilizing polyclonal antibody NIDDK-rLH-S-10, bands at 17 and 19 Kd representing LH subunits were identified. In addition, a high molecular weight 66 Kd band was seen. Surprisingly this high molecular weight band was also seen in rat cerebral cortex, brain stem, hypothalamus, spinal cord, lung, liver, pancreas, spleen, kidney, testis, and serum. Antibody preabsorbed with iodination grade rat LH antigen no longer recognized the 17 and 19 Kd bands in pituitary, but recognized the 66 Kd bands in pituitary and the other tissues examined. Since 66 Kd is the molecular weight of albumin, we found that antisera to rat albumin recognized this same high molecular weight band in the tissues examined. Preabsorption of LH antibody with albumin reduced the ability of that antibody to recognize this 66 Kd. A monoclonal antibody to bovine LH beta-subunit recognized only the LH protein in anterior pituitary, but no high molecular weight band

Original languageEnglish (US)
Pages (from-to)477-491
Number of pages15
JournalEndocrine Research
Volume16
Issue number4
DOIs
StatePublished - Jan 1 1990

    Fingerprint

ASJC Scopus subject areas

  • Endocrinology

Cite this