Cross reactivity of a monoclonal pan T-cell antibody (anti-Leu 4) with prostate epithelium

David Grignon, D. Banerjee

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Cross reactions between monoclonal T-cell antibodies and non-lymphoid tissues have rarely been reported. In this study 28 samples of prostatic tissue obtained at the time of autopsy or surgery, two samples of metastatic prostatic carcinoma and a series of other tumours were snap frozen and sections reacted with a series of monoclonal antibodies directed against the following antigens: Leu 1, Leu 4, T3, T8, T4, T11 and B4. Reactions were detected with an indirect immunofluorescent method. In 10 of 11 normal prostates, 15 of 15 with nodular hyperplasia and 3 of 4 prostatic adenocarcinomas a strong positive reaction occurred with anti-Leu 4. All other antibodies tested were negative. Other tumours tested, including primary carcinomas of lung (2), kidney (3), stomach (1), colon (1), pancreas (1), breast (2), urinary bladder (1), esophagus (1), larynx (1) and malignant melanoma (2), were negative with all antibodies. This is, to our knowledge, the first report of cross reactivity between a monoclonal pan T-cell antibody and epithelium. This cross reaction may be related to a shared antigen between T-cells and prostate epithelial cells or, more likely, it represents reactivity with a shared epitope. Knowledge of this reaction will prevent possible misinterpretations in the evaluation of undifferentiated neoplasms.

Original languageEnglish (US)
Pages (from-to)330-332
Number of pages3
JournalJournal of Urology
Volume137
Issue number2
StatePublished - 1987
Externally publishedYes

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Prostate
Anti-Idiotypic Antibodies
Epithelium
T-Lymphocytes
Antibodies
Cross Reactions
CD5 Antigens
Carcinoma
Neoplasms
Frozen Sections
Larynx
Esophagus
Hyperplasia
Epitopes
Pancreas
Autopsy
Melanoma
Stomach
Colon
Urinary Bladder

ASJC Scopus subject areas

  • Urology

Cite this

Cross reactivity of a monoclonal pan T-cell antibody (anti-Leu 4) with prostate epithelium. / Grignon, David; Banerjee, D.

In: Journal of Urology, Vol. 137, No. 2, 1987, p. 330-332.

Research output: Contribution to journalArticle

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