Cryopreservation of neural stem cells derived from embryonic rats

Zheng wen Ma, Sai li Fu, Lan Yin, Xiao ming Xu, Pei hua Lu

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To establish a cryopreservation method for neural stem cells (NSC) obtained from embryonic rats and to study the viability and differentiation of these cells after cryopreservation. Methods: Stem cells in 10% BSA + 7.5% DMSO were stored in liquid nitrogen. Cell culture and indirect immunofluorescence staining were used to identify the viability, morphology and differentiation of neural stem cells after cryopreservation. Results: Different time of cryopreservation, different passages, different sources of tissue and different trophins did not affect NSC survival (P > 0.05). After cryopreservation, embryonic neural stem cells could survive and expand in vitro for many passages, ahd differentiate into neurons, astrocytes and oligodendrocytes. Conclusion: Cryopreservation and resuscitation did not change biological properties of embryonic NSCs, including their morphology and capacity for expansion and differentiation.

Original languageEnglish (US)
Pages (from-to)122-126
Number of pages5
JournalChinese Journal of Neuroscience
Volume19
Issue number2
StatePublished - Jan 1 2003
Externally publishedYes

Keywords

  • Cryopreservation
  • Embryo
  • Neural stem cell
  • Resuscitation

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology

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