Objective: To establish a cryopreservation method for neural stem cells (NSC) obtained from embryonic rats and to study the viability and differentiation of these cells after cryopreservation. Methods: Stem cells in 10% BSA + 7.5% DMSO were stored in liquid nitrogen. Cell culture and indirect immunofluorescence staining were used to identify the viability, morphology and differentiation of neural stem cells after cryopreservation. Results: Different time of cryopreservation, different passages, different sources of tissue and different trophins did not affect NSC survival (P > 0.05). After cryopreservation, embryonic neural stem cells could survive and expand in vitro for many passages, ahd differentiate into neurons, astrocytes and oligodendrocytes. Conclusion: Cryopreservation and resuscitation did not change biological properties of embryonic NSCs, including their morphology and capacity for expansion and differentiation.
|Original language||English (US)|
|Number of pages||5|
|Journal||Chinese Journal of Neuroscience|
|State||Published - Jan 1 2003|
- Neural stem cell
ASJC Scopus subject areas