Cryopreservation of neural stem cells derived from embryonic rats

Zheng wen Ma, Sai li Fu, Lan Yin, Xiao-Ming Xu, Pei hua Lu

Research output: Contribution to journalArticle

Abstract

Objective: To establish a cryopreservation method for neural stem cells (NSC) obtained from embryonic rats and to study the viability and differentiation of these cells after cryopreservation. Methods: Stem cells in 10% BSA + 7.5% DMSO were stored in liquid nitrogen. Cell culture and indirect immunofluorescence staining were used to identify the viability, morphology and differentiation of neural stem cells after cryopreservation. Results: Different time of cryopreservation, different passages, different sources of tissue and different trophins did not affect NSC survival (P > 0.05). After cryopreservation, embryonic neural stem cells could survive and expand in vitro for many passages, ahd differentiate into neurons, astrocytes and oligodendrocytes. Conclusion: Cryopreservation and resuscitation did not change biological properties of embryonic NSCs, including their morphology and capacity for expansion and differentiation.

Original languageEnglish (US)
Pages (from-to)122-126
Number of pages5
JournalChinese Journal of Neuroscience
Volume19
Issue number2
StatePublished - 2003
Externally publishedYes

Fingerprint

Neural Stem Cells
Cryopreservation
Cell Survival
Oligodendroglia
Embryonic Stem Cells
Indirect Fluorescent Antibody Technique
Dimethyl Sulfoxide
Resuscitation
Astrocytes
Cell Differentiation
Nitrogen
Stem Cells
Cell Culture Techniques
Staining and Labeling
Neurons

Keywords

  • Cryopreservation
  • Embryo
  • Neural stem cell
  • Resuscitation

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)

Cite this

Ma, Z. W., Fu, S. L., Yin, L., Xu, X-M., & Lu, P. H. (2003). Cryopreservation of neural stem cells derived from embryonic rats. Chinese Journal of Neuroscience, 19(2), 122-126.

Cryopreservation of neural stem cells derived from embryonic rats. / Ma, Zheng wen; Fu, Sai li; Yin, Lan; Xu, Xiao-Ming; Lu, Pei hua.

In: Chinese Journal of Neuroscience, Vol. 19, No. 2, 2003, p. 122-126.

Research output: Contribution to journalArticle

Ma, ZW, Fu, SL, Yin, L, Xu, X-M & Lu, PH 2003, 'Cryopreservation of neural stem cells derived from embryonic rats', Chinese Journal of Neuroscience, vol. 19, no. 2, pp. 122-126.
Ma, Zheng wen ; Fu, Sai li ; Yin, Lan ; Xu, Xiao-Ming ; Lu, Pei hua. / Cryopreservation of neural stem cells derived from embryonic rats. In: Chinese Journal of Neuroscience. 2003 ; Vol. 19, No. 2. pp. 122-126.
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