Crystallization and preliminary diffraction studies of a human Fabμ with anti-I activity

A. Cauerhff, I. Polikarpov, I. Mathov, C. Abatangelo, Lilian Plotkin, F. A. Goldbaum, J. Leoni

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

An Fabμ from a human monoclonal cold agglutinin (KAU) was obtained by tryptic digestion and purified to homogeneity by FPLC. Crystals were grown by hanging drop vapour diffusion technique against mother liquor containing Sodium Hepes pH 7.5, and PEG 8000 as precipitant. Crystals were found to belong to the trigonal space group P3121(P3221) with the cell parameters a=b=114.233 Å, c=172.787 Å. The crystals diffracted at least to 2.8 Å resolution at a synchrotron beamline.

Original languageEnglish (US)
Pages (from-to)177-180
Number of pages4
JournalProtein and Peptide Letters
Volume5
Issue number3
StatePublished - 1998
Externally publishedYes

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Synchrotrons
Crystallization
Digestion
Diffraction
Sodium
Crystals
Vapors
cold agglutinins
polyethylene glycol 8000

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Cauerhff, A., Polikarpov, I., Mathov, I., Abatangelo, C., Plotkin, L., Goldbaum, F. A., & Leoni, J. (1998). Crystallization and preliminary diffraction studies of a human Fabμ with anti-I activity. Protein and Peptide Letters, 5(3), 177-180.

Crystallization and preliminary diffraction studies of a human Fabμ with anti-I activity. / Cauerhff, A.; Polikarpov, I.; Mathov, I.; Abatangelo, C.; Plotkin, Lilian; Goldbaum, F. A.; Leoni, J.

In: Protein and Peptide Letters, Vol. 5, No. 3, 1998, p. 177-180.

Research output: Contribution to journalArticle

Cauerhff, A, Polikarpov, I, Mathov, I, Abatangelo, C, Plotkin, L, Goldbaum, FA & Leoni, J 1998, 'Crystallization and preliminary diffraction studies of a human Fabμ with anti-I activity', Protein and Peptide Letters, vol. 5, no. 3, pp. 177-180.
Cauerhff A, Polikarpov I, Mathov I, Abatangelo C, Plotkin L, Goldbaum FA et al. Crystallization and preliminary diffraction studies of a human Fabμ with anti-I activity. Protein and Peptide Letters. 1998;5(3):177-180.
Cauerhff, A. ; Polikarpov, I. ; Mathov, I. ; Abatangelo, C. ; Plotkin, Lilian ; Goldbaum, F. A. ; Leoni, J. / Crystallization and preliminary diffraction studies of a human Fabμ with anti-I activity. In: Protein and Peptide Letters. 1998 ; Vol. 5, No. 3. pp. 177-180.
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