An Fabμ from a human monoclonal cold agglutinin (KAU) was obtained by tryptic digestion and purified to homogeneity by FPLC. Crystals were grown by hanging drop vapour diffusion technique against mother liquor containing Sodium Hepes pH 7.5, and PEG 8000 as precipitant. Crystals were found to belong to the trigonal space group P3121(P3221) with the cell parameters a=b=114.233 Å, c=172.787 Å. The crystals diffracted at least to 2.8 Å resolution at a synchrotron beamline.
|Original language||English (US)|
|Number of pages||4|
|Journal||Protein and Peptide Letters|
|State||Published - Dec 1 1998|
ASJC Scopus subject areas
- Structural Biology