Crystallization and preliminary X-ray investigation of bovine liver mitochondrial aldehyde dehydrogenase

Thomas Hurley, Henry Weiner

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Aldehyde dehydrogenase from bovine liver mitochondria has been crystallized using the sitting drop method of vapor diffusion at 22 °C. The crystals formed from solutions containing, 40 mm-sodium citrate, 1 mm-NAD+ and 21% to 24% polyethylene glycol 3400 (pH 5·3 to 5·5). X-ray diffraction data collected from these crystals indicate that the crystals belong to the orthorhombic space group P212121 with cell dimensions of a = 153·7 A ̊, b = 159·37 A ̊ and c = 101·45 A ̊. The crystals diffract to at least 2·9 Å and a tetramer may comprise the asymmetric unit.

Original languageEnglish
Pages (from-to)1255-1257
Number of pages3
JournalJournal of Molecular Biology
Volume227
Issue number4
DOIs
StatePublished - Oct 20 1992

Fingerprint

Aldehyde Dehydrogenase
Liver Mitochondrion
Crystallization
X-Ray Diffraction
NAD
X-Rays
Liver
Mitochondrial Aldehyde Dehydrogenase
sodium citrate

Keywords

  • aldehyde dehydrogenase
  • crystallization
  • defraction

ASJC Scopus subject areas

  • Virology

Cite this

Crystallization and preliminary X-ray investigation of bovine liver mitochondrial aldehyde dehydrogenase. / Hurley, Thomas; Weiner, Henry.

In: Journal of Molecular Biology, Vol. 227, No. 4, 20.10.1992, p. 1255-1257.

Research output: Contribution to journalArticle

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