Crystallographic and NMR analyses of UvsW and UvsW.1 from bacteriophage T4

Iain D. Kerr, Sivashankar Sivakolundu, Zhenmei Li, Jeffrey C. Buchsbaum, Luke A. Knox, Richard Kriwacki, Stephen W. White

Research output: Contribution to journalArticle

18 Scopus citations


The uvsWXY system is implicated in the replication and repair of the bacteriophage T4 genome. Whereas the roles of the recombinase (UvsX) and the recombination mediator protein (UvsY) are known, the precise role of UvsW is unclear. Sequence analysis identifies UvsW as a member of the monomeric SF2 helicase superfamily that translocates nucleic acid substrates via the action of two RecA-like motor domains. Functional homologies to Escherichia coli RecG and biochemical analyses have shown that UvsW interacts with branched nucleic acid substrates, suggesting roles in recombination and the rescue of stalled replication forks. A sequencing error at the 3′-end of the uvsW gene has revealed a second, short open reading frame that encodes a protein of unknown function called UvsW.1. We have determined the crystal structure of UvsW to 2.7 Å and the NMR solution structure of UvsW.1. UvsW has a four-domain architecture with structural homology to the eukaryotic SF2 helicase, Rad54. A model of the UvsW-ssDNA complex identifies structural elements and conserved residues that may interact with nucleic acid substrates. The NMR solution structure of UvsW.1 reveals a dynamic four-helix bundle with homology to the structure-specific nucleic acid binding module of RecQ helicases.

Original languageEnglish (US)
Pages (from-to)34392-34400
Number of pages9
JournalJournal of Biological Chemistry
Issue number47
StatePublished - Nov 23 2007

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Crystallographic and NMR analyses of UvsW and UvsW.1 from bacteriophage T4'. Together they form a unique fingerprint.

  • Cite this

    Kerr, I. D., Sivakolundu, S., Li, Z., Buchsbaum, J. C., Knox, L. A., Kriwacki, R., & White, S. W. (2007). Crystallographic and NMR analyses of UvsW and UvsW.1 from bacteriophage T4. Journal of Biological Chemistry, 282(47), 34392-34400.