Crystallographic studies of a novel DNA-binding domain from the yeast transcriptional activator Ndt80

Sherwin P. Montano, Michael Pierce, Marie L. Coté, Andrew K. Vershon, Millie Georgiadis

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The Ndt80 protein is a transcriptional activator that plays a key role in the progression of the meiotic divisions in the yeast Saccharomyces cerevisiae. Ndt80 is strongly induced during the middle stages of the sporulation pathway and binds specifically to a promoter element called the MSE to activate transcription of genes required for the meiotic divisions. Here, the preliminary structural and functional studies to characterize the DNA-binding activity of this protein are reported. Through deletion analysis and limited proteolysis studies of Ndt80, a novel 32 kDa DNA-binding domain that is sufficient for DNA-binding in vitro has been defined. Crystals of the DNA-binding domain of Ndt80 in two distinct lattices have been obtained, for which diffraction data extend to 2.3 Å resolution.

Original languageEnglish
Pages (from-to)2127-2130
Number of pages4
JournalActa Crystallographica Section D: Biological Crystallography
Volume58
Issue number12
DOIs
StatePublished - Dec 1 2002

Fingerprint

yeast
Yeast
deoxyribonucleic acid
Yeasts
DNA
division
DNA-Binding Proteins
Proteolysis
proteins
saccharomyces
deletion
Saccharomyces cerevisiae
Transcription
progressions
genes
Proteins
Genes
Diffraction
Crystals
diffraction

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics
  • Condensed Matter Physics
  • Structural Biology

Cite this

Crystallographic studies of a novel DNA-binding domain from the yeast transcriptional activator Ndt80. / Montano, Sherwin P.; Pierce, Michael; Coté, Marie L.; Vershon, Andrew K.; Georgiadis, Millie.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 58, No. 12, 01.12.2002, p. 2127-2130.

Research output: Contribution to journalArticle

Montano, Sherwin P. ; Pierce, Michael ; Coté, Marie L. ; Vershon, Andrew K. ; Georgiadis, Millie. / Crystallographic studies of a novel DNA-binding domain from the yeast transcriptional activator Ndt80. In: Acta Crystallographica Section D: Biological Crystallography. 2002 ; Vol. 58, No. 12. pp. 2127-2130.
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