C/T conversion alters interleukin-1A promoter function in a human astrocyte cell line

Xing Wei, Xianming Chen, Christine Fontanilla, Liming Zhao, Zhong Liang, Richard Dodel, Hampel Hampel, Martin Farlow, Yansheng Du

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Recently, association of an interleukin-1A promoter polymorphism (- 889, thymine/thymine (T/T)) with Alzheimer's disease was reported, suggesting that this cytokine may play an important role in disease development. To understand the mechanism underlying the interleukin-1A promoter's role in Alzheimer's disease, a study comparing promoter function of an interleukin-1A polymorphism was performed in the SVG astroglia cell line. The effects of thymine and cytosine on transcriptional activity of the interleukin-1A promoter were analyzed by testing luciferase-reporter activity in transfected SVG cells. Our results demonstrate that cytosine/thymine conversion increases activity of the interleukin-1A promoter in SVG cells. Both sodium salicylate and lovastatin are able to block induced promoter activities in astroglial cells. Induced promoter activity by the polymorphism (T/T) may result in the upregulation of interleukin-1α protein and "cytokine cycle" amplification, which may promote disease development.

Original languageEnglish (US)
Pages (from-to)1152-1156
Number of pages5
JournalLife Sciences
Volume80
Issue number12
DOIs
StatePublished - Feb 27 2007

    Fingerprint

Keywords

  • A-beta
  • Alzheimer's disease
  • Interleukin-1α
  • LPS
  • Neurodegeneration
  • Neuroinflammation
  • Promoter polymorphism

ASJC Scopus subject areas

  • Pharmacology

Cite this

Wei, X., Chen, X., Fontanilla, C., Zhao, L., Liang, Z., Dodel, R., Hampel, H., Farlow, M., & Du, Y. (2007). C/T conversion alters interleukin-1A promoter function in a human astrocyte cell line. Life Sciences, 80(12), 1152-1156. https://doi.org/10.1016/j.lfs.2006.12.011