C/T conversion alters interleukin-1A promoter function in a human astrocyte cell line

Xing Wei, Xianming Chen, Christine Fontanilla, Liming Zhao, Zhong Liang, Richard Dodel, Hampel Hampel, Martin Farlow, Yansheng Du

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Recently, association of an interleukin-1A promoter polymorphism (- 889, thymine/thymine (T/T)) with Alzheimer's disease was reported, suggesting that this cytokine may play an important role in disease development. To understand the mechanism underlying the interleukin-1A promoter's role in Alzheimer's disease, a study comparing promoter function of an interleukin-1A polymorphism was performed in the SVG astroglia cell line. The effects of thymine and cytosine on transcriptional activity of the interleukin-1A promoter were analyzed by testing luciferase-reporter activity in transfected SVG cells. Our results demonstrate that cytosine/thymine conversion increases activity of the interleukin-1A promoter in SVG cells. Both sodium salicylate and lovastatin are able to block induced promoter activities in astroglial cells. Induced promoter activity by the polymorphism (T/T) may result in the upregulation of interleukin-1α protein and "cytokine cycle" amplification, which may promote disease development.

Original languageEnglish
Pages (from-to)1152-1156
Number of pages5
JournalLife Sciences
Volume80
Issue number12
DOIs
StatePublished - Feb 27 2007

Fingerprint

Thymine
Interleukins
Astrocytes
Cells
Cell Line
Polymorphism
Cytosine
Alzheimer Disease
Cytokines
Sodium Salicylate
Lovastatin
Luciferases
Interleukin-1
Amplification
Up-Regulation
Testing
Proteins

Keywords

  • A-beta
  • Alzheimer's disease
  • Interleukin-1α
  • LPS
  • Neurodegeneration
  • Neuroinflammation
  • Promoter polymorphism

ASJC Scopus subject areas

  • Pharmacology

Cite this

C/T conversion alters interleukin-1A promoter function in a human astrocyte cell line. / Wei, Xing; Chen, Xianming; Fontanilla, Christine; Zhao, Liming; Liang, Zhong; Dodel, Richard; Hampel, Hampel; Farlow, Martin; Du, Yansheng.

In: Life Sciences, Vol. 80, No. 12, 27.02.2007, p. 1152-1156.

Research output: Contribution to journalArticle

Wei, X, Chen, X, Fontanilla, C, Zhao, L, Liang, Z, Dodel, R, Hampel, H, Farlow, M & Du, Y 2007, 'C/T conversion alters interleukin-1A promoter function in a human astrocyte cell line', Life Sciences, vol. 80, no. 12, pp. 1152-1156. https://doi.org/10.1016/j.lfs.2006.12.011
Wei X, Chen X, Fontanilla C, Zhao L, Liang Z, Dodel R et al. C/T conversion alters interleukin-1A promoter function in a human astrocyte cell line. Life Sciences. 2007 Feb 27;80(12):1152-1156. https://doi.org/10.1016/j.lfs.2006.12.011
Wei, Xing ; Chen, Xianming ; Fontanilla, Christine ; Zhao, Liming ; Liang, Zhong ; Dodel, Richard ; Hampel, Hampel ; Farlow, Martin ; Du, Yansheng. / C/T conversion alters interleukin-1A promoter function in a human astrocyte cell line. In: Life Sciences. 2007 ; Vol. 80, No. 12. pp. 1152-1156.
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