Culture of human renal cortex epithelial cells

James A. McAteer, Stephen A. Kempson, Andrew P. Evan

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

A procedure is described for the establishment and propagation of epithelial cell rich cultures derived from normal human kidney cortex (NHK-C cells). Cells are harvested from tissue fragments of donor human kidney by progressive enzymatic dissociation. NHK-C cultures are morphologically heterogeneous but exhibit, predominantly, the functional characteristics of cells of the kidney proximal tubule.

Original languageEnglish (US)
Pages (from-to)143-147
Number of pages5
JournalJournal of tissue culture methods: Tissue Culture Association manual of cell, tissue, and organ culture procedures
Volume13
Issue number3
DOIs
StatePublished - Sep 1991

Keywords

  • cell culture
  • enzymatic dissociation
  • kidney
  • proximal tubule
  • renal cortex

ASJC Scopus subject areas

  • Anatomy
  • Pathology and Forensic Medicine

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