Curcumin protects dopaminergic neuron against LPS induced neurotoxicity in primary rat neuron/glia culture

Sufen Yang, Dan Zhang, Zhengqin Yang, Xiaoming Hu, Steven Qian, Jie Liu, Belinda Wilson, Michelle Block, Jau Shyong Hong

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Using primary rat mesencephalic neuron-glia cultures as an in vitro model of Parkinson's disease (PD), we tested the effect of curcumin, a natural dietary pigment with well-known anti-inflammation effects, on dopaminergic (DA) degeneration. Curcumin pretreatment mitigated LPS-induced DA neurotoxicity in a concentration-dependent manner and curcumin post-treatment also showed protective effect. Microglia depletion abolished this protective effect of curcumin, indicating that microglia play an important role in this effect. Supportively, observation by immunocytochemistry staining using OX-42 antibody showed that curcumin treatment inhibited LPS-induced morphological change of microglia. Besides, LPS-induced production of many proinflammatory factors and their gene expressions decreased dramatically after curcumin treatment. Results also revealed that curcumin treatment decreased LPS-induced activation of two transcription factors-nuclear factors κB (NF-κB) and activator protein-1 (AP-1). Taken together, our study implicated that curcumin might be a potential preventive and therapeutic strategy for inflammation-related neurodegenerative diseases.

Original languageEnglish (US)
Pages (from-to)2044-2053
Number of pages10
JournalNeurochemical Research
Volume33
Issue number10
DOIs
StatePublished - Oct 2008
Externally publishedYes

Fingerprint

Curcumin
Dopaminergic Neurons
Neuroglia
Neurons
Rats
Microglia
Neurodegenerative diseases
Inflammation
Therapeutics
Transcription Factor AP-1
Gene expression
Pigments
Neurodegenerative Diseases
Parkinson Disease
Transcription Factors
Chemical activation
Immunohistochemistry
Observation
Staining and Labeling
Gene Expression

Keywords

  • Curcumin
  • Dopaminergic neuron
  • Microglia
  • Neuroprotection

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

Cite this

Curcumin protects dopaminergic neuron against LPS induced neurotoxicity in primary rat neuron/glia culture. / Yang, Sufen; Zhang, Dan; Yang, Zhengqin; Hu, Xiaoming; Qian, Steven; Liu, Jie; Wilson, Belinda; Block, Michelle; Hong, Jau Shyong.

In: Neurochemical Research, Vol. 33, No. 10, 10.2008, p. 2044-2053.

Research output: Contribution to journalArticle

Yang, Sufen ; Zhang, Dan ; Yang, Zhengqin ; Hu, Xiaoming ; Qian, Steven ; Liu, Jie ; Wilson, Belinda ; Block, Michelle ; Hong, Jau Shyong. / Curcumin protects dopaminergic neuron against LPS induced neurotoxicity in primary rat neuron/glia culture. In: Neurochemical Research. 2008 ; Vol. 33, No. 10. pp. 2044-2053.
@article{ff4ac481ff7840f5bb434d707deb47ea,
title = "Curcumin protects dopaminergic neuron against LPS induced neurotoxicity in primary rat neuron/glia culture",
abstract = "Using primary rat mesencephalic neuron-glia cultures as an in vitro model of Parkinson's disease (PD), we tested the effect of curcumin, a natural dietary pigment with well-known anti-inflammation effects, on dopaminergic (DA) degeneration. Curcumin pretreatment mitigated LPS-induced DA neurotoxicity in a concentration-dependent manner and curcumin post-treatment also showed protective effect. Microglia depletion abolished this protective effect of curcumin, indicating that microglia play an important role in this effect. Supportively, observation by immunocytochemistry staining using OX-42 antibody showed that curcumin treatment inhibited LPS-induced morphological change of microglia. Besides, LPS-induced production of many proinflammatory factors and their gene expressions decreased dramatically after curcumin treatment. Results also revealed that curcumin treatment decreased LPS-induced activation of two transcription factors-nuclear factors κB (NF-κB) and activator protein-1 (AP-1). Taken together, our study implicated that curcumin might be a potential preventive and therapeutic strategy for inflammation-related neurodegenerative diseases.",
keywords = "Curcumin, Dopaminergic neuron, Microglia, Neuroprotection",
author = "Sufen Yang and Dan Zhang and Zhengqin Yang and Xiaoming Hu and Steven Qian and Jie Liu and Belinda Wilson and Michelle Block and Hong, {Jau Shyong}",
year = "2008",
month = "10",
doi = "10.1007/s11064-008-9675-z",
language = "English (US)",
volume = "33",
pages = "2044--2053",
journal = "Neurochemical Research",
issn = "0364-3190",
publisher = "Springer New York",
number = "10",

}

TY - JOUR

T1 - Curcumin protects dopaminergic neuron against LPS induced neurotoxicity in primary rat neuron/glia culture

AU - Yang, Sufen

AU - Zhang, Dan

AU - Yang, Zhengqin

AU - Hu, Xiaoming

AU - Qian, Steven

AU - Liu, Jie

AU - Wilson, Belinda

AU - Block, Michelle

AU - Hong, Jau Shyong

PY - 2008/10

Y1 - 2008/10

N2 - Using primary rat mesencephalic neuron-glia cultures as an in vitro model of Parkinson's disease (PD), we tested the effect of curcumin, a natural dietary pigment with well-known anti-inflammation effects, on dopaminergic (DA) degeneration. Curcumin pretreatment mitigated LPS-induced DA neurotoxicity in a concentration-dependent manner and curcumin post-treatment also showed protective effect. Microglia depletion abolished this protective effect of curcumin, indicating that microglia play an important role in this effect. Supportively, observation by immunocytochemistry staining using OX-42 antibody showed that curcumin treatment inhibited LPS-induced morphological change of microglia. Besides, LPS-induced production of many proinflammatory factors and their gene expressions decreased dramatically after curcumin treatment. Results also revealed that curcumin treatment decreased LPS-induced activation of two transcription factors-nuclear factors κB (NF-κB) and activator protein-1 (AP-1). Taken together, our study implicated that curcumin might be a potential preventive and therapeutic strategy for inflammation-related neurodegenerative diseases.

AB - Using primary rat mesencephalic neuron-glia cultures as an in vitro model of Parkinson's disease (PD), we tested the effect of curcumin, a natural dietary pigment with well-known anti-inflammation effects, on dopaminergic (DA) degeneration. Curcumin pretreatment mitigated LPS-induced DA neurotoxicity in a concentration-dependent manner and curcumin post-treatment also showed protective effect. Microglia depletion abolished this protective effect of curcumin, indicating that microglia play an important role in this effect. Supportively, observation by immunocytochemistry staining using OX-42 antibody showed that curcumin treatment inhibited LPS-induced morphological change of microglia. Besides, LPS-induced production of many proinflammatory factors and their gene expressions decreased dramatically after curcumin treatment. Results also revealed that curcumin treatment decreased LPS-induced activation of two transcription factors-nuclear factors κB (NF-κB) and activator protein-1 (AP-1). Taken together, our study implicated that curcumin might be a potential preventive and therapeutic strategy for inflammation-related neurodegenerative diseases.

KW - Curcumin

KW - Dopaminergic neuron

KW - Microglia

KW - Neuroprotection

UR - http://www.scopus.com/inward/record.url?scp=51749116969&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=51749116969&partnerID=8YFLogxK

U2 - 10.1007/s11064-008-9675-z

DO - 10.1007/s11064-008-9675-z

M3 - Article

C2 - 18368483

AN - SCOPUS:51749116969

VL - 33

SP - 2044

EP - 2053

JO - Neurochemical Research

JF - Neurochemical Research

SN - 0364-3190

IS - 10

ER -