Curcumin protects dopaminergic neuron against LPS induced neurotoxicity in primary rat neuron/glia culture

Sufen Yang, Dan Zhang, Zhengqin Yang, Xiaoming Hu, Steven Qian, Jie Liu, Belinda Wilson, Michelle Block, Jau Shyong Hong

Research output: Contribution to journalArticle

61 Scopus citations

Abstract

Using primary rat mesencephalic neuron-glia cultures as an in vitro model of Parkinson's disease (PD), we tested the effect of curcumin, a natural dietary pigment with well-known anti-inflammation effects, on dopaminergic (DA) degeneration. Curcumin pretreatment mitigated LPS-induced DA neurotoxicity in a concentration-dependent manner and curcumin post-treatment also showed protective effect. Microglia depletion abolished this protective effect of curcumin, indicating that microglia play an important role in this effect. Supportively, observation by immunocytochemistry staining using OX-42 antibody showed that curcumin treatment inhibited LPS-induced morphological change of microglia. Besides, LPS-induced production of many proinflammatory factors and their gene expressions decreased dramatically after curcumin treatment. Results also revealed that curcumin treatment decreased LPS-induced activation of two transcription factors-nuclear factors κB (NF-κB) and activator protein-1 (AP-1). Taken together, our study implicated that curcumin might be a potential preventive and therapeutic strategy for inflammation-related neurodegenerative diseases.

Original languageEnglish (US)
Pages (from-to)2044-2053
Number of pages10
JournalNeurochemical Research
Volume33
Issue number10
DOIs
StatePublished - Oct 1 2008

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Keywords

  • Curcumin
  • Dopaminergic neuron
  • Microglia
  • Neuroprotection

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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