Cure with low-dose total-body irradiation of the hematological disorder induced in mice with the Friend virus: Possible mechanism involving interferon-γ and Interleukin-2

R. N. Shen, L. Lu, G. S. Feng, J. Miller, M. W. Taylor, H. E. Broxmeyer

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

The effects of split low-dose total-body irradiation (TBI; 150 cGy) on production of interferon (IFN)-γ and Interleukin-2 (IL-2) and on the growth characteristics of erythroid progenitor cells (BFU-E) have been assessed in normal mice, normal mice receiving TBI only, mice infected with the polycythemia-inducing strain of the Friend virus complex (FVC-P), and FVC-P infected mice receiving 150 cGy TBI on days 5 and 12. It was found that lymphocytes from the spleens of TBI-treated mice previously infected with FVC-P produced in response to phytohemagglutinin (PHA) and concanavalin A (Con A) stimulation up to 15 times greater amounts of IFN-γ than cells from untreated FVC-P-infected mice. IL-2 production in Con A-stimulated spleen cell cultures also increased when cells were isolated from FVC-P-infected mice treated by low-dose TBI compared to untreated FVC-P-infected mice. TBI treatment was associated with > 99% ablation of 'erythropoietin-independent' BFU-E colony formation. The results suggest that the cure of FVC-P-infected mice by low-dose TBI may result from activation of the IFN-γ system and IL-2 production.

Original languageEnglish (US)
Pages (from-to)105-109
Number of pages5
JournalLymphokine and Cytokine Research
Volume10
Issue number1-2
StatePublished - Jan 1 1991

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ASJC Scopus subject areas

  • Immunology
  • Hematology

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