Cure with low-dose total-body irradiation of the hematological disorder induced in mice with the Friend virus: Possible mechanism involving interferon-γ and Interleukin-2

R. N. Shen, L. Lu, G. S. Feng, J. Miller, M. W. Taylor, Hal Broxmeyer

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The effects of split low-dose total-body irradiation (TBI; 150 cGy) on production of interferon (IFN)-γ and Interleukin-2 (IL-2) and on the growth characteristics of erythroid progenitor cells (BFU-E) have been assessed in normal mice, normal mice receiving TBI only, mice infected with the polycythemia-inducing strain of the Friend virus complex (FVC-P), and FVC-P infected mice receiving 150 cGy TBI on days 5 and 12. It was found that lymphocytes from the spleens of TBI-treated mice previously infected with FVC-P produced in response to phytohemagglutinin (PHA) and concanavalin A (Con A) stimulation up to 15 times greater amounts of IFN-γ than cells from untreated FVC-P-infected mice. IL-2 production in Con A-stimulated spleen cell cultures also increased when cells were isolated from FVC-P-infected mice treated by low-dose TBI compared to untreated FVC-P-infected mice. TBI treatment was associated with > 99% ablation of 'erythropoietin-independent' BFU-E colony formation. The results suggest that the cure of FVC-P-infected mice by low-dose TBI may result from activation of the IFN-γ system and IL-2 production.

Original languageEnglish
Pages (from-to)105-109
Number of pages5
JournalLymphokine and Cytokine Research
Volume10
Issue number1-2
StatePublished - 1991

Fingerprint

Friend murine leukemia virus
Whole-Body Irradiation
Interferons
Interleukin-2
Erythroid Precursor Cells
Concanavalin A
Spleen
Polycythemia
Phytohemagglutinins
Erythropoietin
Cell Culture Techniques
Lymphocytes

ASJC Scopus subject areas

  • Immunology
  • Hematology

Cite this

Cure with low-dose total-body irradiation of the hematological disorder induced in mice with the Friend virus : Possible mechanism involving interferon-γ and Interleukin-2. / Shen, R. N.; Lu, L.; Feng, G. S.; Miller, J.; Taylor, M. W.; Broxmeyer, Hal.

In: Lymphokine and Cytokine Research, Vol. 10, No. 1-2, 1991, p. 105-109.

Research output: Contribution to journalArticle

@article{8fab0bdefd5b4d298f794e32bd0c0e5d,
title = "Cure with low-dose total-body irradiation of the hematological disorder induced in mice with the Friend virus: Possible mechanism involving interferon-γ and Interleukin-2",
abstract = "The effects of split low-dose total-body irradiation (TBI; 150 cGy) on production of interferon (IFN)-γ and Interleukin-2 (IL-2) and on the growth characteristics of erythroid progenitor cells (BFU-E) have been assessed in normal mice, normal mice receiving TBI only, mice infected with the polycythemia-inducing strain of the Friend virus complex (FVC-P), and FVC-P infected mice receiving 150 cGy TBI on days 5 and 12. It was found that lymphocytes from the spleens of TBI-treated mice previously infected with FVC-P produced in response to phytohemagglutinin (PHA) and concanavalin A (Con A) stimulation up to 15 times greater amounts of IFN-γ than cells from untreated FVC-P-infected mice. IL-2 production in Con A-stimulated spleen cell cultures also increased when cells were isolated from FVC-P-infected mice treated by low-dose TBI compared to untreated FVC-P-infected mice. TBI treatment was associated with > 99{\%} ablation of 'erythropoietin-independent' BFU-E colony formation. The results suggest that the cure of FVC-P-infected mice by low-dose TBI may result from activation of the IFN-γ system and IL-2 production.",
author = "Shen, {R. N.} and L. Lu and Feng, {G. S.} and J. Miller and Taylor, {M. W.} and Hal Broxmeyer",
year = "1991",
language = "English",
volume = "10",
pages = "105--109",
journal = "Journal of Interferon and Cytokine Research",
issn = "1079-9907",
publisher = "Mary Ann Liebert Inc.",
number = "1-2",

}

TY - JOUR

T1 - Cure with low-dose total-body irradiation of the hematological disorder induced in mice with the Friend virus

T2 - Possible mechanism involving interferon-γ and Interleukin-2

AU - Shen, R. N.

AU - Lu, L.

AU - Feng, G. S.

AU - Miller, J.

AU - Taylor, M. W.

AU - Broxmeyer, Hal

PY - 1991

Y1 - 1991

N2 - The effects of split low-dose total-body irradiation (TBI; 150 cGy) on production of interferon (IFN)-γ and Interleukin-2 (IL-2) and on the growth characteristics of erythroid progenitor cells (BFU-E) have been assessed in normal mice, normal mice receiving TBI only, mice infected with the polycythemia-inducing strain of the Friend virus complex (FVC-P), and FVC-P infected mice receiving 150 cGy TBI on days 5 and 12. It was found that lymphocytes from the spleens of TBI-treated mice previously infected with FVC-P produced in response to phytohemagglutinin (PHA) and concanavalin A (Con A) stimulation up to 15 times greater amounts of IFN-γ than cells from untreated FVC-P-infected mice. IL-2 production in Con A-stimulated spleen cell cultures also increased when cells were isolated from FVC-P-infected mice treated by low-dose TBI compared to untreated FVC-P-infected mice. TBI treatment was associated with > 99% ablation of 'erythropoietin-independent' BFU-E colony formation. The results suggest that the cure of FVC-P-infected mice by low-dose TBI may result from activation of the IFN-γ system and IL-2 production.

AB - The effects of split low-dose total-body irradiation (TBI; 150 cGy) on production of interferon (IFN)-γ and Interleukin-2 (IL-2) and on the growth characteristics of erythroid progenitor cells (BFU-E) have been assessed in normal mice, normal mice receiving TBI only, mice infected with the polycythemia-inducing strain of the Friend virus complex (FVC-P), and FVC-P infected mice receiving 150 cGy TBI on days 5 and 12. It was found that lymphocytes from the spleens of TBI-treated mice previously infected with FVC-P produced in response to phytohemagglutinin (PHA) and concanavalin A (Con A) stimulation up to 15 times greater amounts of IFN-γ than cells from untreated FVC-P-infected mice. IL-2 production in Con A-stimulated spleen cell cultures also increased when cells were isolated from FVC-P-infected mice treated by low-dose TBI compared to untreated FVC-P-infected mice. TBI treatment was associated with > 99% ablation of 'erythropoietin-independent' BFU-E colony formation. The results suggest that the cure of FVC-P-infected mice by low-dose TBI may result from activation of the IFN-γ system and IL-2 production.

UR - http://www.scopus.com/inward/record.url?scp=0025817753&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025817753&partnerID=8YFLogxK

M3 - Article

C2 - 1908321

AN - SCOPUS:0025817753

VL - 10

SP - 105

EP - 109

JO - Journal of Interferon and Cytokine Research

JF - Journal of Interferon and Cytokine Research

SN - 1079-9907

IS - 1-2

ER -