Cutting edge

Differential requirements for Stat4 in expression of glycosyltransferases responsible for selectin ligand formation in Th1 cells

S. J. White, G. H. Underhill, Mark Kaplan, G. S. Kansas

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

A role for Stat4 in IL-12-induced up-regulation of selectin ligands on Th1 cells was explored. Th1 cells generated from Stat4-/- mice exhibited no IL-12-inducible P-selectin ligands, no up-regulation of core 2 β1,6-glucosaminyltransferase I (C2GlcNAcT-I), and low levels of the Th1 transcription factor T-bet. In contrast, Stat4-/- Th1 cells exhibited only a partial defect in expression of IL-12-inducible E-selectin ligands and expressed equivalently high levels of α1,3-fucosyltransferase VII (FucT-VII) as wild-type Th1 cells. FucT-VII expression was induced by T cell activation, and was enhanced by IL-12 independently of Stat4, whereas C2GlcNAcT-I up-regulation was mediated exclusively by IL-12, acting through Stat4. These data show that FucT-VII and C2GlcNAcT-I are controlled through distinct pathways and imply the existence of at least one other IL-12-inducible glycosyltransferase required for E-selectin and possibly P-selectin ligand formation in Th1 cells.

Original languageEnglish (US)
Pages (from-to)628-631
Number of pages4
JournalJournal of Immunology
Volume167
Issue number2
StatePublished - Jul 15 2001
Externally publishedYes

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Selectins
Glycosyltransferases
Th1 Cells
galactoside 3-fucosyltransferase
Interleukin-12
Ligands
P-Selectin
E-Selectin
Up-Regulation
T-Lymphocytes
beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-acetylglucosaminyl transferase

ASJC Scopus subject areas

  • Immunology

Cite this

Cutting edge : Differential requirements for Stat4 in expression of glycosyltransferases responsible for selectin ligand formation in Th1 cells. / White, S. J.; Underhill, G. H.; Kaplan, Mark; Kansas, G. S.

In: Journal of Immunology, Vol. 167, No. 2, 15.07.2001, p. 628-631.

Research output: Contribution to journalArticle

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