Cutting edge

Mouse pellino-2 modulates IL-1 and lipopolysaccharide signaling

Kang Yeol Yu, Hyung Joo Kwon, David A M Norman, Eva Vig, Mark Goebl, Maureen Harrington

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Pellino is a Drosophila protein originally isolated in a two-hybrid screen for proteins interacting with the serine/threonine kinase, pelle. Although mammalian homologs have been identified in mouse and man, the function of pellino is as yet unknown. In this study, the cloning, expression pattern, and a preliminary characterization of mouse pellino-2 is described. These studies reveal that mouse pellino-2 is expressed during embryogenesis and in a tissue-restricted manner in the adult. IL-1 induces the association of mouse pellino-2 with the mouse pelle-like kinase/IL-1R-associated kinase protein, a mammalian homolog of pelle. Ectopic pellino-2 expression did not result in NF-κB activation. However, ectopic expression of a mouse pellino-2 antisense construct inhibited IL-1 or LPS-induced activation of NF-κB-dependent IL-8 promoter activity. Our data reveal that mouse pellino-2 is a tissue-restricted component of a signaling pathway that couples the mouse pelle-like kinase/IL-1R-associated kinase protein to IL-1- or LPS-dependent signaling.

Original languageEnglish
Pages (from-to)4075-4078
Number of pages4
JournalJournal of Immunology
Volume169
Issue number8
StatePublished - Oct 15 2002

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Interleukin-1
Lipopolysaccharides
Protein Kinases
Phosphotransferases
Protein-Serine-Threonine Kinases
Interleukin-8
Embryonic Development
Organism Cloning
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Cutting edge : Mouse pellino-2 modulates IL-1 and lipopolysaccharide signaling. / Yu, Kang Yeol; Kwon, Hyung Joo; Norman, David A M; Vig, Eva; Goebl, Mark; Harrington, Maureen.

In: Journal of Immunology, Vol. 169, No. 8, 15.10.2002, p. 4075-4078.

Research output: Contribution to journalArticle

Yu, Kang Yeol ; Kwon, Hyung Joo ; Norman, David A M ; Vig, Eva ; Goebl, Mark ; Harrington, Maureen. / Cutting edge : Mouse pellino-2 modulates IL-1 and lipopolysaccharide signaling. In: Journal of Immunology. 2002 ; Vol. 169, No. 8. pp. 4075-4078.
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