Cutting edge

NADPH oxidase modulates MHC class II antigen presentation by B cells

Victoria L. Crotzer, Juan D. Matute, Andrés A. Arias, Heng Zhao, Lawrence Quilliam, Mary C. Dinauer, Janice Blum

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Phagocyte NADPH oxidase plays a key role in pathogen clearance via reactive oxygen species (ROS) production. Defects in oxidase function result in chronic granulomatous disease with hallmark recurrent microbial infections and inflammation. The oxidase's role in the adaptive immune response is not well understood. Class II presentation of cytoplasmic and exogenous Ag to CD4+ T cells was impaired in human B cells with reduced oxidase p40phox subunit expression. Naturally arising mutations, which compromise p40phox function in a chronic granulomatous disease patient, also perturbed class II Ag presentation and intracellular ROS production. Reconstitution of patient B cells with a wild-type, but not a mutant, p40phox allele restored exogenous Ag presentation and intracellular ROS generation. Remarkably, class II presentation of epitopes from membrane Ag was robust in p40phox-deficient B cells. These studies reveal a role for NADPH oxidase and p40phox in skewing epitope selection and T cell recognition of self Ag.

Original languageEnglish
Pages (from-to)3800-3804
Number of pages5
JournalJournal of Immunology
Volume189
Issue number8
DOIs
StatePublished - Oct 15 2012

Fingerprint

NADPH Oxidase
Histocompatibility Antigens Class II
Antigen Presentation
B-Lymphocytes
Chronic Granulomatous Disease
Reactive Oxygen Species
Oxidoreductases
T-Lymphocyte Epitopes
Adaptive Immunity
Phagocytes
Epitopes
Alleles
neutrophil cytosol factor 40K
Inflammation
T-Lymphocytes
Mutation
Membranes
Infection

ASJC Scopus subject areas

  • Immunology

Cite this

Cutting edge : NADPH oxidase modulates MHC class II antigen presentation by B cells. / Crotzer, Victoria L.; Matute, Juan D.; Arias, Andrés A.; Zhao, Heng; Quilliam, Lawrence; Dinauer, Mary C.; Blum, Janice.

In: Journal of Immunology, Vol. 189, No. 8, 15.10.2012, p. 3800-3804.

Research output: Contribution to journalArticle

Crotzer, Victoria L. ; Matute, Juan D. ; Arias, Andrés A. ; Zhao, Heng ; Quilliam, Lawrence ; Dinauer, Mary C. ; Blum, Janice. / Cutting edge : NADPH oxidase modulates MHC class II antigen presentation by B cells. In: Journal of Immunology. 2012 ; Vol. 189, No. 8. pp. 3800-3804.
@article{0a9a09a0c7d441318e02f1e2218b9a6c,
title = "Cutting edge: NADPH oxidase modulates MHC class II antigen presentation by B cells",
abstract = "Phagocyte NADPH oxidase plays a key role in pathogen clearance via reactive oxygen species (ROS) production. Defects in oxidase function result in chronic granulomatous disease with hallmark recurrent microbial infections and inflammation. The oxidase's role in the adaptive immune response is not well understood. Class II presentation of cytoplasmic and exogenous Ag to CD4+ T cells was impaired in human B cells with reduced oxidase p40phox subunit expression. Naturally arising mutations, which compromise p40phox function in a chronic granulomatous disease patient, also perturbed class II Ag presentation and intracellular ROS production. Reconstitution of patient B cells with a wild-type, but not a mutant, p40phox allele restored exogenous Ag presentation and intracellular ROS generation. Remarkably, class II presentation of epitopes from membrane Ag was robust in p40phox-deficient B cells. These studies reveal a role for NADPH oxidase and p40phox in skewing epitope selection and T cell recognition of self Ag.",
author = "Crotzer, {Victoria L.} and Matute, {Juan D.} and Arias, {Andr{\'e}s A.} and Heng Zhao and Lawrence Quilliam and Dinauer, {Mary C.} and Janice Blum",
year = "2012",
month = "10",
day = "15",
doi = "10.4049/jimmunol.1103080",
language = "English",
volume = "189",
pages = "3800--3804",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "8",

}

TY - JOUR

T1 - Cutting edge

T2 - NADPH oxidase modulates MHC class II antigen presentation by B cells

AU - Crotzer, Victoria L.

AU - Matute, Juan D.

AU - Arias, Andrés A.

AU - Zhao, Heng

AU - Quilliam, Lawrence

AU - Dinauer, Mary C.

AU - Blum, Janice

PY - 2012/10/15

Y1 - 2012/10/15

N2 - Phagocyte NADPH oxidase plays a key role in pathogen clearance via reactive oxygen species (ROS) production. Defects in oxidase function result in chronic granulomatous disease with hallmark recurrent microbial infections and inflammation. The oxidase's role in the adaptive immune response is not well understood. Class II presentation of cytoplasmic and exogenous Ag to CD4+ T cells was impaired in human B cells with reduced oxidase p40phox subunit expression. Naturally arising mutations, which compromise p40phox function in a chronic granulomatous disease patient, also perturbed class II Ag presentation and intracellular ROS production. Reconstitution of patient B cells with a wild-type, but not a mutant, p40phox allele restored exogenous Ag presentation and intracellular ROS generation. Remarkably, class II presentation of epitopes from membrane Ag was robust in p40phox-deficient B cells. These studies reveal a role for NADPH oxidase and p40phox in skewing epitope selection and T cell recognition of self Ag.

AB - Phagocyte NADPH oxidase plays a key role in pathogen clearance via reactive oxygen species (ROS) production. Defects in oxidase function result in chronic granulomatous disease with hallmark recurrent microbial infections and inflammation. The oxidase's role in the adaptive immune response is not well understood. Class II presentation of cytoplasmic and exogenous Ag to CD4+ T cells was impaired in human B cells with reduced oxidase p40phox subunit expression. Naturally arising mutations, which compromise p40phox function in a chronic granulomatous disease patient, also perturbed class II Ag presentation and intracellular ROS production. Reconstitution of patient B cells with a wild-type, but not a mutant, p40phox allele restored exogenous Ag presentation and intracellular ROS generation. Remarkably, class II presentation of epitopes from membrane Ag was robust in p40phox-deficient B cells. These studies reveal a role for NADPH oxidase and p40phox in skewing epitope selection and T cell recognition of self Ag.

UR - http://www.scopus.com/inward/record.url?scp=84867286629&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84867286629&partnerID=8YFLogxK

U2 - 10.4049/jimmunol.1103080

DO - 10.4049/jimmunol.1103080

M3 - Article

VL - 189

SP - 3800

EP - 3804

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 8

ER -