Cutting edge

Rho activation and actin polarization are dependent on plexin-A1 in dendritic cells

So Young Eun, Brian P. O'Connor, Athena W. Wong, Hendrick W. Van Deventer, Debra J. Taxman, William Reed, Ping Li, Janice Blum, Karen P. McKinnon, Jenny P Y Ting

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We recently identified expression of the semaphorin receptor, plexin-A1, in dendritic cells (DCs); however, its function in these cells remains to be elucidated. To investigate function and maximize physiological relevance, we devised a retroviral approach to ablate plexin-A1 gene expression using small hairpin RNA (shRNA) in primary bone marrow-derived DCs. We show that plexin-A1 localizes within the cytoplasm of immature DCs, becomes membrane-associated, and is enriched at the immune synapse in mature DCs, Reducing plexin-A1 expression with shRNA greatly reduced actin polarization as well as Rho activation without affecting Rac or Cdc42 activation. A Rho inhibitor, C3, also reduced actin polarization. These changes were accompanied by the near-ablation of T cell activation. We propose a mechanism of adaptive immune regulation in which plexin-A1 controls Rho activation and actin cytoskeletal rearrangements in DCs that is associated with enhanced DC-T cell interactions.

Original languageEnglish
Pages (from-to)4271-4275
Number of pages5
JournalJournal of Immunology
Volume177
Issue number7
StatePublished - Oct 1 2006

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Dendritic Cells
Actins
Small Interfering RNA
Semaphorins
T-Lymphocytes
Cell Communication
Synapses
plexin
Cytoplasm
Bone Marrow
Cell Membrane
Gene Expression

ASJC Scopus subject areas

  • Immunology

Cite this

Eun, S. Y., O'Connor, B. P., Wong, A. W., Van Deventer, H. W., Taxman, D. J., Reed, W., ... Ting, J. P. Y. (2006). Cutting edge: Rho activation and actin polarization are dependent on plexin-A1 in dendritic cells. Journal of Immunology, 177(7), 4271-4275.

Cutting edge : Rho activation and actin polarization are dependent on plexin-A1 in dendritic cells. / Eun, So Young; O'Connor, Brian P.; Wong, Athena W.; Van Deventer, Hendrick W.; Taxman, Debra J.; Reed, William; Li, Ping; Blum, Janice; McKinnon, Karen P.; Ting, Jenny P Y.

In: Journal of Immunology, Vol. 177, No. 7, 01.10.2006, p. 4271-4275.

Research output: Contribution to journalArticle

Eun, SY, O'Connor, BP, Wong, AW, Van Deventer, HW, Taxman, DJ, Reed, W, Li, P, Blum, J, McKinnon, KP & Ting, JPY 2006, 'Cutting edge: Rho activation and actin polarization are dependent on plexin-A1 in dendritic cells', Journal of Immunology, vol. 177, no. 7, pp. 4271-4275.
Eun SY, O'Connor BP, Wong AW, Van Deventer HW, Taxman DJ, Reed W et al. Cutting edge: Rho activation and actin polarization are dependent on plexin-A1 in dendritic cells. Journal of Immunology. 2006 Oct 1;177(7):4271-4275.
Eun, So Young ; O'Connor, Brian P. ; Wong, Athena W. ; Van Deventer, Hendrick W. ; Taxman, Debra J. ; Reed, William ; Li, Ping ; Blum, Janice ; McKinnon, Karen P. ; Ting, Jenny P Y. / Cutting edge : Rho activation and actin polarization are dependent on plexin-A1 in dendritic cells. In: Journal of Immunology. 2006 ; Vol. 177, No. 7. pp. 4271-4275.
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