Cutting edge: Rho activation and actin polarization are dependent on plexin-A1 in dendritic cells

So Young Eun, Brian P. O'Connor, Athena W. Wong, Hendrick W. Van Deventer, Debra J. Taxman, William Reed, Ping Li, Janice S. Blum, Karen P. McKinnon, Jenny P.Y. Ting

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

We recently identified expression of the semaphorin receptor, plexin-A1, in dendritic cells (DCs); however, its function in these cells remains to be elucidated. To investigate function and maximize physiological relevance, we devised a retroviral approach to ablate plexin-A1 gene expression using small hairpin RNA (shRNA) in primary bone marrow-derived DCs. We show that plexin-A1 localizes within the cytoplasm of immature DCs, becomes membrane-associated, and is enriched at the immune synapse in mature DCs, Reducing plexin-A1 expression with shRNA greatly reduced actin polarization as well as Rho activation without affecting Rac or Cdc42 activation. A Rho inhibitor, C3, also reduced actin polarization. These changes were accompanied by the near-ablation of T cell activation. We propose a mechanism of adaptive immune regulation in which plexin-A1 controls Rho activation and actin cytoskeletal rearrangements in DCs that is associated with enhanced DC-T cell interactions.

Original languageEnglish (US)
Pages (from-to)4271-4275
Number of pages5
JournalJournal of Immunology
Volume177
Issue number7
DOIs
StatePublished - Oct 1 2006

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Eun, S. Y., O'Connor, B. P., Wong, A. W., Van Deventer, H. W., Taxman, D. J., Reed, W., Li, P., Blum, J. S., McKinnon, K. P., & Ting, J. P. Y. (2006). Cutting edge: Rho activation and actin polarization are dependent on plexin-A1 in dendritic cells. Journal of Immunology, 177(7), 4271-4275. https://doi.org/10.4049/jimmunol.177.7.4271