CXCR4 pathway associated with family history of melanoma

Wen Qing Li, Jiali Han, Hans R. Widlund, Mick Correll, Yaoyu E. Wang, John Quackenbush, Martin C. Mihm, Alvaro Laga Canales, Shaowei Wu, Todd Golub, Yujin Hoshida, David J. Hunter, George Murphy, Thomas S. Kupper, Abrar A. Qureshi

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Purpose: Genetic predisposition plays a major role in the etiology of melanoma, but known genetic markers only account for a limited fraction of family-history-associated melanoma cases. Expression microarrays have offered the opportunity to identify further genomic profiles correlated with family history of melanoma. We aimed to distinguish mRNA expression signatures between melanoma cases with and without a family history of melanoma. Methods: Based on the Nurses' Health Study, family history was defined as having one or more first-degree family members diagnosed with melanoma. Melanoma diagnosis was confirmed by reviewing pathology reports, and tumor blocks were collected by mail from across the USA. Genomic interrogation was accomplished through evaluating expression profiling of formalin-fixed paraffin-embedded tissues from 78 primary cutaneous invasive melanoma cases, on either a 6K or whole-genome (24K) Illumina gene chip. Gene set enrichment analysis was performed for each batch to determine the differentially enriched pathways and key contributing genes. Results: The CXC chemokine receptor 4 (CXCR4) pathway was consistently up-regulated within cases of familial melanoma in both platforms. Leading edge analysis showed four genes from the CXCR4 pathway, including MAPK1, PLCG1, CRK, and PTK2, were among the core members that contributed to the enrichment of this pathway. There was no association between the enrichment of CXCR4 pathway and NRAS, BRAF mutation, or Breslow thickness of the primary melanoma cases. Conclusions: We found that the CXCR4 pathway might constitute a novel susceptibility pathway associated with family history of melanoma in first-degree relatives.

Original languageEnglish (US)
Pages (from-to)125-132
Number of pages8
JournalCancer Causes and Control
Volume25
Issue number1
DOIs
StatePublished - Jan 1 2014

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Keywords

  • Genetic pathway analysis
  • Genome-wide expression profiling
  • Melanoma

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Li, W. Q., Han, J., Widlund, H. R., Correll, M., Wang, Y. E., Quackenbush, J., Mihm, M. C., Canales, A. L., Wu, S., Golub, T., Hoshida, Y., Hunter, D. J., Murphy, G., Kupper, T. S., & Qureshi, A. A. (2014). CXCR4 pathway associated with family history of melanoma. Cancer Causes and Control, 25(1), 125-132. https://doi.org/10.1007/s10552-013-0315-9