Cyclic GMP injected into retinal rod outer segments increases latency and amplitude of response to illumination

Grant Nicol, W. H. Miller

Research output: Contribution to journalArticle

113 Citations (Scopus)

Abstract

The authors have injected cyclic GMP intracellularly by iontophoresis through the recording electrode into single rod outer segments of the isolated superfused retina of the toad, Bufo marinus. The two most marked effects of the injection are: (i) the latency of the hyperpolarizing membrane-potential change caused by illumination is increased from 5 to 50 times normal, the increase in latency being inversely proportional to the light stimulus intensity; and (ii) the amplitude of the hyperpolarizing receptor potential is increased. These effects are reversible. The authors findings are consistent with the hypothesis that cyclic GMP is a link in the molecular chain of events that controls the inward flow of sodium ions in light and darkness. The increased latency the authors observe after injection of cyclic GMP may be caused by a time delay necessary for light-activated phosphodiesterase to hydrolyze the excess cyclic GMP. By this reasoning, the excess cyclic GMP perpetuates the dark current long after illumination. Excitation may be controlled by cyclic GMP if, as our experiments suggest, its hydrolysis initiates the hyperpolarizing receptor potential.

Original languageEnglish (US)
Pages (from-to)5217-5220
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume75
Issue number10
StatePublished - 1978
Externally publishedYes

Fingerprint

Retinal Photoreceptor Cell Outer Segment
Rod Cell Outer Segment
Retinal Rod Photoreceptor Cells
Cyclic GMP
Lighting
Reaction Time
Light
Bufo marinus
Iontophoresis
Injections
Darkness
Phosphoric Diester Hydrolases
Anura
Membrane Potentials
Retina
Electrodes
Hydrolysis
Sodium
Ions

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

@article{5e37943aa2494ffeb9c2fb16b1dee160,
title = "Cyclic GMP injected into retinal rod outer segments increases latency and amplitude of response to illumination",
abstract = "The authors have injected cyclic GMP intracellularly by iontophoresis through the recording electrode into single rod outer segments of the isolated superfused retina of the toad, Bufo marinus. The two most marked effects of the injection are: (i) the latency of the hyperpolarizing membrane-potential change caused by illumination is increased from 5 to 50 times normal, the increase in latency being inversely proportional to the light stimulus intensity; and (ii) the amplitude of the hyperpolarizing receptor potential is increased. These effects are reversible. The authors findings are consistent with the hypothesis that cyclic GMP is a link in the molecular chain of events that controls the inward flow of sodium ions in light and darkness. The increased latency the authors observe after injection of cyclic GMP may be caused by a time delay necessary for light-activated phosphodiesterase to hydrolyze the excess cyclic GMP. By this reasoning, the excess cyclic GMP perpetuates the dark current long after illumination. Excitation may be controlled by cyclic GMP if, as our experiments suggest, its hydrolysis initiates the hyperpolarizing receptor potential.",
author = "Grant Nicol and Miller, {W. H.}",
year = "1978",
language = "English (US)",
volume = "75",
pages = "5217--5220",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "10",

}

TY - JOUR

T1 - Cyclic GMP injected into retinal rod outer segments increases latency and amplitude of response to illumination

AU - Nicol, Grant

AU - Miller, W. H.

PY - 1978

Y1 - 1978

N2 - The authors have injected cyclic GMP intracellularly by iontophoresis through the recording electrode into single rod outer segments of the isolated superfused retina of the toad, Bufo marinus. The two most marked effects of the injection are: (i) the latency of the hyperpolarizing membrane-potential change caused by illumination is increased from 5 to 50 times normal, the increase in latency being inversely proportional to the light stimulus intensity; and (ii) the amplitude of the hyperpolarizing receptor potential is increased. These effects are reversible. The authors findings are consistent with the hypothesis that cyclic GMP is a link in the molecular chain of events that controls the inward flow of sodium ions in light and darkness. The increased latency the authors observe after injection of cyclic GMP may be caused by a time delay necessary for light-activated phosphodiesterase to hydrolyze the excess cyclic GMP. By this reasoning, the excess cyclic GMP perpetuates the dark current long after illumination. Excitation may be controlled by cyclic GMP if, as our experiments suggest, its hydrolysis initiates the hyperpolarizing receptor potential.

AB - The authors have injected cyclic GMP intracellularly by iontophoresis through the recording electrode into single rod outer segments of the isolated superfused retina of the toad, Bufo marinus. The two most marked effects of the injection are: (i) the latency of the hyperpolarizing membrane-potential change caused by illumination is increased from 5 to 50 times normal, the increase in latency being inversely proportional to the light stimulus intensity; and (ii) the amplitude of the hyperpolarizing receptor potential is increased. These effects are reversible. The authors findings are consistent with the hypothesis that cyclic GMP is a link in the molecular chain of events that controls the inward flow of sodium ions in light and darkness. The increased latency the authors observe after injection of cyclic GMP may be caused by a time delay necessary for light-activated phosphodiesterase to hydrolyze the excess cyclic GMP. By this reasoning, the excess cyclic GMP perpetuates the dark current long after illumination. Excitation may be controlled by cyclic GMP if, as our experiments suggest, its hydrolysis initiates the hyperpolarizing receptor potential.

UR - http://www.scopus.com/inward/record.url?scp=0018088939&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018088939&partnerID=8YFLogxK

M3 - Article

C2 - 217019

AN - SCOPUS:0018088939

VL - 75

SP - 5217

EP - 5220

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 10

ER -